R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
ENPP1 (E4I2P) Rabbit mAb #40036
Filter:
- WB
Supporting Data
| REACTIVITY | M R |
| SENSITIVITY | Endogenous |
| MW (kDa) | 110 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
Species Cross-Reactivity Key:
- M-Mouse
- R-Rat
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
ENPP1 (E4I2P) Rabbit mAb recognizes endogenous levels of total ENPP1 protein.
Species Reactivity:
Mouse, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu281 of mouse ENPP1 protein.
Background
Ectonucleotide pyrophosphatase-phosphodiesterase 1 (ENPP1) is a single-pass, type II transmembrane protein primarily involved in ATP hydrolysis at the plasma membrane. Targeting of ENPP1 to the basolateral cell surface relies on the presence of a carboxy-terminal di-leucine-based signal (1). ENPP1 plays important roles in bone mineralization and soft tissue calcification (2-5). Mutations in the corresponding ENPP1 gene cause generalized arterial calcification in infancy (GACI) and idiopathic infantile arterial calcification (IIAC) (6,7). ENPP1 inhibits insulin receptor function and overexpression of this enzyme causes insulin resistance and glucose intolerance in mice (8,9). Genetic variants of ENPP1 have been associated with obesity and type 2 diabetes (10-12).
- Bello, V. et al. (2001) Mol Biol Cell 12, 3004-15.
- Okawa, A. et al. (1998) Nat Genet 19, 271-3.
- Nakamura, I. et al. (1999) Hum Genet 104, 492-7.
- Harmey, D. et al. (2004) Am J Pathol 164, 1199-209.
- Hessle, L. et al. (2002) Proc Natl Acad Sci USA 99, 9445-9.
- Rutsch, F. et al. (2003) Nat Genet 34, 379-81.
- Ruf, N. et al. (2005) Hum Mutat 25, 98.
- Dong, H. et al. (2005) Diabetes 54, 367-72.
- Maddux, B.A. et al. (2006) Am J Physiol Endocrinol Metab 290, E746-9.
- Meyre, D. et al. (2005) Nat Genet 37, 863-7.
- Keene, K.L. et al. (2008) Diabetes 57, 1057-62.
- Bacci, S. et al. (2007) Curr Opin Clin Nutr Metab Care 10, 403-9.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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