Enolase-1 Antibody #3810
Filter:
- WB
- IP
Supporting Data
| REACTIVITY | H M R Mk |
| SENSITIVITY | Endogenous |
| MW (kDa) | 47 |
| SOURCE | Rabbit |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
- Mk-Monkey
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
Enolase-1 Antibody detects endogenous levels of total enolase-1 protein and does not corss-react with enolase-2.
Species Reactivity:
Human, Mouse, Rat, Monkey
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the sequence of human enolase-1. Antibodies are purified by protein A and peptide affinity chromatography.
Background
Enolase is an important glycolytic enzyme involved in the interconversion of 2-phosphoglycerate to phosphoenolpyruvate. Mammalian enolase exists as three subunits: enolase-1 (α-enolase), enolase-2 (γ-enolase) and enolase-3 (β-enolase) that can form both homo- and heterodimers. Expression of the enolase isoforms differs in a tissue specific manner (1). Enolase-1 plays a key role in anaerobic metabolism under hypoxic conditions and may act as a cell surface plasminogen receptor during tissue invasion (2,3). Abnormal expression of enolase-1 is associated with tumor progression in some cases of breast and lung cancer (4-7). Alternatively, an enolase-1 splice variant (MBP-1) binds the c-myc promoter p2 and may function as a tumor suppressor. For this reason enolase-1 is considered as a potential therapeutic target in the treatment of some forms of cancer (8).
- Pancholi, V. (2001) Cell Mol Life Sci 58, 902-20.
- Redlitz, A. et al. (1995) Eur J Biochem 227, 407-15.
- Jiang, B.H. et al. (1997) Cancer Res 57, 5328-35.
- Peebles, K.A. et al. (2003) Carcinogenesis 24, 651-7.
- Zhang, L. et al. (2000) J Surg Res 93, 108-19.
- Wu, W. et al. (2002) Clin Exp Metastasis 19, 319-26.
- Hennipman, A. et al. (1988) Tumour Biol 9, 241-8.
- Feo, S. et al. (2000) FEBS Lett 473, 47-52.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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