R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
ELF3 (F8J2G) Rabbit mAb #84039
Filter:
- WB
- IP
- IF
- ChIP
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | 42 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
- IF-Immunofluorescence
- ChIP-Chromatin Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
For optimal ChIP results, use 10 μL of antibody and 10 μg of chromatin (approximately 4 × 106 cells) per IP. This antibody has been validated using SimpleChIP® Enzymatic Chromatin IP Kits.
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:200 |
| Immunofluorescence (Immunocytochemistry) | 1:800 - 1:3200 |
| Chromatin IP | 1:50 |
| Chromatin IP-seq | 1:50 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
ELF3 (F8J2G) Rabbit mAb recognizes endogenous levels of total ELF3 protein.
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human ELF3 protein.
Background
The E26 transformation-specific (ETS) transcription factors bind to a core 5’-GGAA/T-3’ DNA motif and are known to regulate many biological processes, including cell proliferation, differentiation, apoptosis, hematopoiesis, and angiogenesis; they have also been described in pathological conditions, including cancer (reviewed in 1). ETS transcription factor E74-like factor 3 (ELF3), also called ESE-1, ESX, Jen, and ERT, belongs to a subtype of ETS proteins characterized by a predominant epithelial-specific expression profile (2-6). ELF3 shows widespread expression across tissues with high expression in the digestive tract. Targeted knockout of the ELF3 gene in mice results in lethality in about 30% of the embryos to E11.5, along with abnormalities in the intestinal epithelium (7). ELF3 can have dualistic roles in cancer with tumor-promoting and suppressing activity (reviewed in 8,9). Dysregulation of ELF3 through genetic and epigenetic mechanisms such as inactivating mutations, promoter methylation, and gene amplification have been reported across multiple cancer types and stages of disease. In addition, ELF3 has been shown to play a role in immune disorders and inflammation (10-12).
- Wang, Y. et al. (2023) Biochim Biophys Acta Rev Cancer 1878, 188872.
- Chang, C.H. et al. (1997) Oncogene 14, 1617-22.
- Oettgen, P. et al. (1997) Mol Cell Biol 17, 4419-33.
- Tymms, M.J. et al. (1997) Oncogene 15, 2449-62.
- Andreoli, J.M. et al. (1997) Nucleic Acids Res 25, 4287-95.
- Chang, J. et al. (2000) Oncogene 19, 151-4.
- Ng, A.Y. et al. (2002) Gastroenterology 122, 1455-66.
- Luk, I.Y. et al. (2018) Molecules23, 2191, doi: 10.3390/molecules23092191.
- Ju, Y. et al. (2024) Life Sci 346, 122637.
- Grall, F. et al. (2003) Arthritis Rheum 48, 1249-60.
- Brown, C. et al. (2004) J Biol Chem 279, 12794-803.
- Kushwah, R. et al. (2011) J Immunol 187, 4639-53.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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