eIF4G Antibody #2498
Filter:
- WB
- IHC
- IF
- F
Supporting Data
| REACTIVITY | H M R Mk |
| SENSITIVITY | Endogenous |
| MW (kDa) | 220 |
| SOURCE | Rabbit |
Application Key:
- WB-Western Blotting
- IHC-Immunohistochemistry
- IF-Immunofluorescence
- F-Flow Cytometry
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
- Mk-Monkey
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunohistochemistry (Paraffin) | 1:100 |
| Immunofluorescence (Immunocytochemistry) | 1:200 |
| Flow Cytometry (Fixed/Permeabilized) | 1:50 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
eIF4G Antibody detects endogenous levels of total eIF4G protein.
Species Reactivity:
Human, Mouse, Rat, Monkey
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to a sequence of human eIF4G. Antibodies are purified by protein A and peptide affinity chromatography.
Background
The initiation of translation is an important biological event and a variety of factors contribute to this process. Members of the eIF4 translation initiation factor family bind to the 5' m7GTP mRNA cap and unwind the mRNA secondary structure (1,2). The amino-terminal portion of eIF4G physically associates with eIF4E to stimulate the binding of eIF4E to the mRNA cap structure (3). eIF4G also interacts with eIF3 and eIF4A and serves as an adaptor molecule in the eIF4 complex (4). Moreover, eIF4G plays a role in internal ribosomal entry site (IRES)-mediated initiation of translation (5,6). The eIF4G family includes eIF4G1 (eIF4GI), eIF4G2 (p97, DAP5 or NAT1), and eIF4G3 (eIF4GII) (7). These factors share a homologous sequence that provides for interaction with initiation factors eIF3 and eIF4A. Both eIF4G1 and eIF4G3 are involved in cap-dependent translation, while eIF4G2 plays a role in IRES-mediated translation of some genes during cell stress (7,8).
- Yan, R. and Rhoads, R.E. (1995) Genomics 26, 394-398.
- Morley, S.J. et al. (1997) RNA 3, 1085-1104.
- Haghighat, A. and Sonenberg, N. (1997) J. Biol. Chem. 272, 21677-21680.
- De Gregorio, E. et al. (1998) RNA 4, 828-836.
- Ohlmann, T. et al. (1996) EMBO J. 15, 1371-1382.
- Borman, A.M. and Kean, K.M. (1997) Virology 237, 129-136.
- Henis-Korenblit, S. et al. (2002) Proc. Natl. Acad. Sci. USA 99, 5400-5405.
- Nevins, T.A. et al. (2003) J. Biol. Chem. 278, 3572-3579.
限制使用
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