R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
eIF4A2 (F3S3X) Rabbit mAb #41385
Filter:
- WB
- IP
Supporting Data
| REACTIVITY | H M R Mk |
| SENSITIVITY | Endogenous |
| MW (kDa) | 48 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
- Mk-Monkey
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
eIF4A2 (F3S3X) Rabbit mAb recognizes endogenous levels of total eIF4A2 protein. This antibody does not cross-react with eIF4A1 or eIF4A3 proteins.
Species Reactivity:
Human, Mouse, Rat, Monkey
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding His12 of human eIF4A2 protein.
Background
A variety of factors contribute to the important biological event of translation initiation. The eukaryotic initiation factor 4F (eIF4F) complex of translation initiation factors binds to the 5' m7 GTP cap to open up the mRNA secondary structure and allow small ribosome subunit binding (1). eIF4A, an eIF4 complex component that acts as an ATP-dependent RNA helicase, unwinds the secondary structure of the 5' mRNA untranslated region to mediate ribosome binding (2,3). In addition, eIF4A has recently been shown to repress Dpp/BMP signaling in a translation-independent manner in Drosophila (4,5).
eIF4A members (eIF4A1/2/3) are highly homologous proteins with non-redundant functions. eIF4A1 and eIF4A2 function within the cytoplasm, and their expression is correlated, whereas eIF4A3 functions in the nucleus as part of the exon junction complex (EJC) (6,7). eIF4A2 activity is critical in the functional gene regulation of microRNAs (8). eIF4A2 is capable of inhibiting deadenylation of mRNAs via CNOT7 inhibition (9). During stress response, eIF4A2 is sequestered into granules through Lys226 sumoylation (10). eIF4A2 plays an active role in promoting viral life cycle (11,12). Higher expression of eIF4A2 has been shown in different cancer types including esophageal squamous cell carcinoma and colorectal cancer (13,14).
eIF4A members (eIF4A1/2/3) are highly homologous proteins with non-redundant functions. eIF4A1 and eIF4A2 function within the cytoplasm, and their expression is correlated, whereas eIF4A3 functions in the nucleus as part of the exon junction complex (EJC) (6,7). eIF4A2 activity is critical in the functional gene regulation of microRNAs (8). eIF4A2 is capable of inhibiting deadenylation of mRNAs via CNOT7 inhibition (9). During stress response, eIF4A2 is sequestered into granules through Lys226 sumoylation (10). eIF4A2 plays an active role in promoting viral life cycle (11,12). Higher expression of eIF4A2 has been shown in different cancer types including esophageal squamous cell carcinoma and colorectal cancer (13,14).
- Rogers, G.W. et al. (2001) J. Biol. Chem. 276, 12598-12608.
- Rogers, G.W. et al. (1999) J. Biol. Chem. 274, 12236-12244.
- Svitkin, Y.V. et al. (2001) RNA 7, 382-394.
- Li, J. and Li, W.X. (2006) Nat. Cell Biol. 8, 1407-1414.
- Affolter, M. and Pyrowolakis, G. (2006) Nat. Cell Biol. 8, 1319-1321.
- Lu, W.T. et al. (2014) Biochem Soc Trans 42, 166-72.
- Galicia-Vázquez, G. et al. (2012) RNA 18, 1373-84.
- Meijer, H.A. et al. (2013) Science 340, 82-5.
- Meijer, H.A. et al. (2019) Nucleic Acids Res 47, 8224-8238.
- Jongjitwimol, J. et al. (2016) J Cell Sci 129, 2407-15.
- Ndzinu, J.K. et al. (2018) Microbes Infect 20, 346-352.
- Song, Z. et al. (2019) Res Vet Sci 123, 39-46.
- Chen, Z.H. et al. (2019) J Exp Clin Cancer Res 38, 196.
- Lyu, S. et al. (2020) Oncol Lett 20, 177.
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