eIF2β Antibody #50537
Filter:
- WB
Supporting Data
| REACTIVITY | H M R Mk |
| SENSITIVITY | Endogenous |
| MW (kDa) | 48 |
| SOURCE | Rabbit |
Application Key:
- WB-Western Blotting
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
- Mk-Monkey
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, and 50% glycerol. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
eIF2β Antibody recognizes endogenous levels of total eIF2β protein.
Species Reactivity:
Human, Mouse, Rat, Monkey
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro207 of human eIF2β protein. Antibodies are purified by peptide affinity chromatography.
Background
eIF2 binds GTP and Met-tRNAi and transfers Met-tRNA to the 40S subunit to form the 43S preinitiation complex (1,2). eIF2 promotes a new round of translation initiation by exchanging GDP for GTP, a reaction catalyzed by eIF2β (1,2). Kinases that are activated by viral infection (PKR), endoplasmic reticulum stress (PERK/PEK), amino acid deprivation (GCN2), or heme deficiency (HRI) can phosphorylate the α subunit of eIF2 (3,4). This phosphorylation stabilizes the eIF2-GDP-eIF2β complex and inhibits the turnover of eIF2β. Induction of PKR by IFN-γ and TNF-α induces potent phosphorylation of eIF2α at Ser51 (5,6).
The eIF2β subunit plays a major role in various interactions of the eIF2 trimer with other proteins. eIF2β has been shown to directly interact with other eIF factors, including eIF5, eIF2B, and eIF3 (7-9). eIF2β has also been shown to directly bind mRNA through a C-terminal zinc-finger motif (10,11). Phosphorylation of eIF2β by CK2 promotes the guanine nucleotide exchange activity of eIF2β (12,13).
The eIF2β subunit plays a major role in various interactions of the eIF2 trimer with other proteins. eIF2β has been shown to directly interact with other eIF factors, including eIF5, eIF2B, and eIF3 (7-9). eIF2β has also been shown to directly bind mRNA through a C-terminal zinc-finger motif (10,11). Phosphorylation of eIF2β by CK2 promotes the guanine nucleotide exchange activity of eIF2β (12,13).
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- Llorens, F. et al. (2003) Biochem J 375, 623-31.
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限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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