EGF Receptor (E746-A750del Specific) (D6B6) XP^® Rabbit mAb #2085

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Supporting Data

REACTIVITY	H
SENSITIVITY	Endogenous
MW (kDa)	175
Source/Isotype	Rabbit IgG

Application Key:

WB-Western Blotting
IP-Immunoprecipitation
IHC-Immunohistochemistry
IF-Immunofluorescence
F-Flow Cytometry

Species Cross-Reactivity Key:

H-Human

Product Information

Product Usage Information

Application	Dilution
Western Blotting	1:1000
Immunoprecipitation	1:100
Immunohistochemistry (Paraffin)	1:100 - 1:400
Immunofluorescence (Immunocytochemistry)	1:100 - 1:200
Flow Cytometry (Fixed/Permeabilized)	1:250 - 1:1000

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

For a carrier free (BSA and azide free) version of this product see product #53177.

Protocol

Specificity / Sensitivity

EGF Receptor (E746-A750del Specific) (D6B6) XP^® Rabbit mAb detects endogenous levels of EGFR E746-A750del mutant protein.

Species Reactivity:

Human

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to E746-A750del mutant sequence of human EGFR.

Background

The epidermal growth factor (EGF) receptor is a 170 kDa transmembrane tyrosine kinase that belongs to the HER/ErbB protein family. Research studies have shown that somatic mutations in the tyrosine kinase domain of EGF receptor (EGFR) are present in a subset of lung adenocarcinomas that respond to EGFR inhibitors, such as gefitinib and erlotinib (1-3). Two types of mutations account for approximately 90% of mutated cases: a specific point mutation, L858R, that occurs in exon 21 and short in-frame deletions in exon 19 (4,5). The most frequent exon 19 deletion is E746-A750, accounting for 90% of lesions at this site, although some rare variants occur.

Database Links

UniProt ID: P00533

Entrez-Gene Id: 1956

限制使用

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For Research Use Only. Not For Use In Diagnostic Procedures.

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EGF Receptor (E746-A750del Specific) (D6B6) XP® Rabbit mAb #2085