eEF2k Antibody #3692
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Western blot analysis of extracts from C6, 293, HeLa and SW-13 cells using eEF2k Antibody.
Supporting Data
| REACTIVITY | H R Mk |
| SENSITIVITY | Endogenous |
| MW (kDa) | 105 |
| SOURCE | Rabbit |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
- IF-Immunofluorescence
Species Cross-Reactivity Key:
- H-Human
- R-Rat
- Mk-Monkey
- Related Products
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:200 |
| Immunofluorescence (Immunocytochemistry) | 1:100 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
eEF2k Antibody detects endogenous levels of total eEF2k independent of phosphorylation.
Species Reactivity:
Human, Rat, Monkey
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding the N-terminus of human eEF2k. Antibodies are purified by protein A and peptide affinity chromatography.
Background
Eukaryotic elongation factor 2 kinase (eEF2k) phosphorylates and inactivates eEF2, resulting in the inhibition of peptide-chain elongation (1). eEF2k is normally dependent on Ca2+ ions and calmodulin (2,3). It can be activated by PKA in response to elevated cAMP levels (4-6), which are generally increased in stress- or starvation-related conditions. eEF2k can also be regulated in response to a wide range of stimuli that promote cell growth and protein synthesis. This involves the phosphorylation of eEF2k by p90RSK and p70 S6 kinase at Ser366 or by SAPK4/p38delta at Ser359, leading to the inactivation of eEF2k (7,8), which facilitates the dephosphorylation of eEF2, and thus promotes translation.
- Ryazanov, A.G. et al. (1997) Proc. Natl. Acad. Sci. USA 94, 4884-4889.
- Nairn, A.C. et al. (1985) Proc. Natl. Acad. Sci. USA 82, 7839-7943.
- Palfrey, H.C. et al. (1987) J. Biol. Chem. 262, 9785-9792.
- Redpath, N.T. et al. (1993) Biochem. J. 293, 31-34.
- Diggle, T.A. et al. (1998) Biochem. J. 336, 525-529.
- Hovland, R. et al. (1999) FEBS Lett. 444, 97-101.
- Wang, X. et al. (2001) EMBO J. 20, 4370-4379.
- Knebel, A. et al. (2001) EMBO J. 20, 4360-4369.
Pathways
Explore pathways related to this product.
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