R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
E4BP4/NFIL3 (D5K8O) Rabbit mAb #14312
Filter:
- WB
- IP
Supporting Data
| REACTIVITY | H M R |
| SENSITIVITY | Endogenous |
| MW (kDa) | 60 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:100 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
E4BP4/NFIL3 (D5K8O) Rabbit mAb recognizes endogenous levels of total E4BP4/NFIL3 protein.
Species Reactivity:
Human, Mouse, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro455 of human E4BP4/NFIL3 protein.
Background
E4BP4/NFIL3 is a basic leucine zipper transcriptional regulator that plays a variety of roles in the immune system (1). For example, E4BP4/NFIL3 is required for bone marrow-derived NK cell development (2,3). In addition, it regulates IgE class switching in B cells by controlling IL-4 dependent induction of germ-line ε (GLε) transcription (4). In macrophages, E4BP4/NFIL3 is induced following exposure to bacteria and negatively regulates transcription of IL-12 p40. E4BP4/NFIL3 also controls cytokine production by T cells. Th2 cells from E4BP4/NFIL3(-/-) mice produced elevated levels of IL-5 an IL-13, but reduced levels of IL-4 ad IL-10 (5,6). Chronically stimulated Th1 cells, regulatory T cells, and NKT cells from E4BP4/NFIL3(-/-) mice all produce reduced levels of IL-10 and IL-13 (6). Finally, E4BP4/NFIL3 is also required for the development of conventional CD8α+ dendritic cells, as this subset is absent from E4BP4/NFIL3(-/-) mice (7).
- Cowell, I.G. et al. (1992) Mol Cell Biol 12, 3070-7.
- Gascoyne, D.M. et al. (2009) Nat Immunol 10, 1118-24.
- Seillet, C. et al. (2014) J Immunol 192, 2667-76.
- Kashiwada, M. et al. (2010) Proc Natl Acad Sci U S A 107, 821-6.
- Kashiwada, M. et al. (2011) EMBO J 30, 2071-82.
- Motomura, Y. et al. (2011) Nat Immunol 12, 450-9.
- Kashiwada, M. et al. (2011) Blood 117, 6193-7.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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