R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
E2F4 (E3G2G) Rabbit mAb #40291
Filter:
- WB
- IP
- ChIP
Supporting Data
| REACTIVITY | H M R Mk |
| SENSITIVITY | Endogenous |
| MW (kDa) | 62 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
- ChIP-Chromatin Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
- Mk-Monkey
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
| Chromatin IP | 1:50 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
E2F4 (E3G2G) Rabbit mAb recognizes endogenous levels of total E2F4 protein.
Species Reactivity:
Human, Mouse, Rat, Monkey
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu405 of human E2F4 protein.
Background
The E2F family consists of 8 transcription factors that regulate genes that control cell cycle progression by complexing with DP and Rb proteins (1-4). E2F transcriptional activation is generally opposed by associating with RB proteins, pRB, p107, and p130 (5-7). E2F-1, -2, and -3a function as activators that can help quiescent cells enter S phase, while E2F-3b, -4, and -5 repress cell growth through the recruitment of HDAC’s and other corepressors to target genes (8-10). E2F-6 diverges considerably from other family members, and has repressive properties governed not through interaction with Rb proteins, but by recruiting the polycomb repressive complex (11,12). E2F-7, and -8 are unique in that they have two DNA-binding domains and do not heterodimerize with DP proteins. These E2F family members repress transcription and delay progression of the cell cycle through the regulation of E2F-1 (13-15)
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- Dyson, N. (1998) Genes Dev 12, 2245-62.
- DeGregori, J. et al. (1997) Proc Natl Acad Sci U S A 94, 7245-50.
- Lukas, J. et al. (1996) Mol Cell Biol 16, 1047-57.
- Meloni, A.R. et al. (1999) Proc Natl Acad Sci U S A 96, 9574-9.
- Trimarchi, J.M. et al. (1998) Proc Natl Acad Sci U S A 95, 2850-5.
- Trimarchi, J.M. et al. (2001) Proc Natl Acad Sci U S A 98, 1519-24.
- Logan, N. et al. (2004) Oncogene 23, 5138-50.
- Logan, N. et al. (2005) Oncogene 24, 5000-4.
- Moon, N.S. and Dyson, N. (2008) Dev Cell 14, 1-3.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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