Render Target: SSR
Render Timestamp: 2025-01-09T19:30:47.184Z
Commit: 199712eb9daea12d88cc0e67894a8a09f475f8cb
XML generation date: 2024-05-10 22:31:35.974
Product last modified at: 2025-01-01T09:02:28.576Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

Dynactin p150Glued (D1W1O) Rabbit mAb #69399

Filter:
  • WB
  • IP
  • IHC

    Supporting Data

    REACTIVITY H M R
    SENSITIVITY Endogenous
    MW (kDa) 150
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    • IHC-Immunohistochemistry 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • R-Rat 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:50
    Immunohistochemistry (Paraffin) 1:100

    Protocol

    Specificity / Sensitivity

    Dynactin p150Glued (D1W1O) Rabbit mAb recognizes endogenous levels of total dynactin p150Glued protein. Based on the amino acid sequence of the peptide antigen used, this antibody is expected to detect all isoforms of dynactin p150Glued, including p135.

    Species Reactivity:

    Human, Mouse, Rat

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val1206 of human dynactin p150Glued protein.

    Background

    Cytoplasmic dynein is a multi-subunit motor complex that regulates microtubule organization as well as the transport and positioning of organelles. Dynactin is a multi-subunit dynein-activating complex, which regulates the interaction of the dynein motor with various cellular cargoes, and enhances dynein’s processivity. p150Glued/DCTN1/Dynactin 1 is the largest subunit of the dynactin complex (1-3). In mitosis, cytoplasmic dynein regulates spindle organization, chromosome movement and centrosome separation (4). The dynactin subunit p150Glued is phosphorylated at serine 19 by the mitotic kinase aurora A during anaphase, and this phosphorylation is required for the appropriate regulation of spindle assembly (5). In neurons, axonal transport is important for cellular function and survival. Dysfunction and mutations in dynein and dynactin subunits, including p150Glued, have been linked to human neurodegenerative diseases such as Alzheimer’s Disease (6-7), Perry Syndrome (8) and ALS (9).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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