R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
DTX3L (D5F2J) Rabbit mAb #14795
Filter:
- WB
- IP
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | 84 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
DTX3L (D5F2J) Rabbit mAb recognizes endogenous levels of total DTX3L protein.
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val22 of human DTX3L protein.
Background
The E3 ubiquitin-protein ligase DTX3L (BBAP) is a deltex (DTX) family ligase that binds B aggressive lymphoma 1 (BAL1) to mediate the monoubiquitination of histone H4 Lys91 in response to DNA damage (1,2). The early recruitment of poly-ADP-ribose polymerase 1 (PARP1) protein to sites of DNA damage leads to poly-ADP-ribosylation (PARylation) of multiple target proteins. This results in the subsequent recruitment of DTX3L/BAL1 through binding of the BAL macrodomain to PARylated proteins. DTX3L/BAL1 then mediates the monoubiquitination of histone H4 Lys91 that is required for subsequent methylation of histone H4 Lys20 and recruitment of p53-binding protein 1 (1-3). DTX3L and BAL1 are highly expressed in malignant B-cell lymphomas and act to confer chemotherapy-resistance by protecting cells from DNA damaging agents (4).
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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