dsRNA (K1) Mouse mAb #28764
Filter:
- IF
Supporting Data
| REACTIVITY | All |
| SENSITIVITY | Endogenous |
| MW (kDa) | |
| Source/Isotype | Mouse IgG2a kappa |
Application Key:
- IF-Immunofluorescence
Species Cross-Reactivity Key:
- All-All Species Expected
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Immunofluorescence (Immunocytochemistry) | 1:800 - 1:3200 |
Storage
Supplied in a PBS solution that does not contain any stabilizers or preservatives and has been lyophilized. The lyophilized antibody is stable for 24 months when stored at -20°C. Reconstitute using 200 μl of sterile distilled water to create a 1 mg/ml solution. A slight precipitate may be present, but will not interfere with antibody performance. Recommended to centrifuge the reconstituted antibody and use the supernatant. Once reconstituted, this product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles.
Protocol
Specificity / Sensitivity
dsRNA (K1) Mouse mAb recognizes dsRNA where the length of the helix is greater than or equal to 40 base pairs (bp). The ability to recognize dsRNA is independent of the sequence and nucleotide composition of the antigen. The K1 clone shows a higher affinity to poly(I:C) than to other dsRNA antigens (6).
Species Reactivity:
All Species Expected
Source / Purification
Monoclonal antibody is produced by immunizing animals with a nucleic acid sequence.
Background
Double-stranded RNA (dsRNA) is produced during the replication cycle of a broad range of viruses and can trigger the innate immune response. dsRNA is present if cells are infected with a dsRNA virus or it can be generated during the course of single-stranded RNA (ssRNA) virus replication. Host cells do not produce dsRNA so the innate immune response can differentiate between host and viral RNAs by the presence of dsRNA (1). The innate immune response is controlled by several classes of germline-encoded pattern-recognition receptors (PRRs), including retinoic acid-inducible gene-I (RIG-I)-like receptors (RLRs), Toll-like receptors (TLRs), and NOD-like receptors (NLRs) that sense viral components, such as dsRNA, ssRNA, and DNA (2,3). Activation of PRRs by dsRNA leads to production of type I interferons (IFNs) and proinflammatory cytokines, triggering the adaptive immune response (2,3). Understanding how antiviral responses are initiated, the counter strategies that viruses adopt, and exploring the viral life cycle gives researchers a better comprehension of how to treat infections of different viral diseases (4,5).
- Ogden, K.M. and Prasad, B.V. (2015) Oncotarget 6, 28535-6.
- Cui, J. et al. (2014) Hum Vaccin Immunother 10, 3270-85.
- Takeuchi, O. and Akira, S. (2009) Immunol Rev 227, 75-86.
- Knoops, K. et al. (2012) J Virol 86, 2474-87.
- Welsch, S. et al. (2009) Cell Host Microbe 5, 365-75.
- Schönborn, J. et al. (1991) Nucleic Acids Res 19, 2993-3000.
限制使用
除非 CST 的合法授书代表以书面形式书行明确同意，否书以下条款适用于 CST、其关书方或分书商提供的书品。 任何书充本条款或与本条款不同的客书条款和条件，除非书 CST 的合法授书代表以书面形式书独接受， 否书均被拒书，并且无效。
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For Research Use Only. Not For Use In Diagnostic Procedures.
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This product is a SCICONS brand antibody manufactured by Absolute Biotech.
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