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Doublecortin Antibody

Doublecortin Antibody #4604

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Supporting Data

REACTIVITY	M R
SENSITIVITY	Endogenous
MW (kDa)
SOURCE	Rabbit

Application Key:

IF-Immunofluorescence

Species Cross-Reactivity Key:

M-Mouse
R-Rat

Product Information

Product Usage Information

Application	Dilution
Immunofluorescence (Frozen)	1:800

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

Doublecortin Antibody detects endogenous levels of total doublecortin protein. Nonspecific labeling of fixed frozen mouse pancreas, colon, small intestine, retina, and cell bodies in adult brain are observed by immunofluorescence.

For superior performance in tissue, Doublecortin (F6K9E) Rabbit mAb #56130 is recommended.

Species Reactivity:

Mouse, Rat

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to human doublecortin. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Mutations in doublecortin (DCX) cause Lissencephaly (smooth brain), a neuronal migration disorder characterized by epilepsy and mental retardation (1). Doublecortin is a microtubule-associated protein that stabilizes and bundles microtubules. A conserved doublecortin domain mediates the interaction with microtubules, and interestingly most missense mutations cluster in this domain (2). Kinases JNK, CDK5, and PKA phosphorylate doublecortin. JNK phosphorylates Thr321, Thr331, and Ser334 while PKA phosphorylates Ser47 and CDK5 phosphorylates Ser297 (3-5). Phosphorylation of Ser297 lowers the affinity of doublecortin to microtubules. Furthermore, mutations of Ser297 result in migration defects (5).

Database Links

UniProt ID: O43602

Entrez-Gene Id: 1641

限制使用

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For Research Use Only. Not For Use In Diagnostic Procedures.

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