DOG1/Anoctamin 1 (K9) Mouse mAb #54598
Filter:
- IHC
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | |
| Source/Isotype | Mouse IgG2b |
Application Key:
- IHC-Immunohistochemistry
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| IHC Leica Bond | 1:50 - 1:200 |
| Immunohistochemistry (Paraffin) | 1:50 - 1:200 |
Storage
Supplied as liquid tissue culture supernatant containing sodium azide as a preservative. Stable for 6 months when stored at 4°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
DOG1/Anoctamin 1 (K9) Mouse mAb recognizes endogenous levels of DOG1/Anoctamin 1 protein.
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a recombinant protein corresponding to the carboxy terminus of human DOG1/Anoctamin 1 protein.
Background
Anoctamin 1 is a plasma membrane calcium-activated chloride channel, also known as discovered on gastrointestinal stromal tumors protein 1 (DOG1) or transmembrane protein 16A (TMEM16A) (1). It is essential for chloride secretion from epithelial tissues (2) and has been shown to be a specific marker for cells of Cajal (3), the pacemaker cells that control smooth muscle contraction. Anoctamin 1 knockout mice exhibit an altered gastric smooth muscle rhythmic contraction (4). More recently, research studies have identified anoctamin 1 as a heat sensor in nociceptive neurons (5). Heat above 44ºC triggers anoctamin 1-dependent depolarization, contributing to the mediation of thermal nociception (5).
- Schroeder, B.C. et al. (2008) Cell 134, 1019-29.
- Thomas-Gatewood, C. et al. (2011) Am J Physiol Heart Circ Physiol 301, H1819-27.
- Gomez-Pinilla, P.J. et al. (2009) Am J Physiol Gastrointest Liver Physiol 296, G1370-81.
- Hwang, S.J. et al. (2009) J Physiol 587, 4887-904.
- Cho, H. et al. (2012) Nat Neurosci 15, 1015-21.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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