Render Target: SSR
Render Timestamp:
4/3/2025, 8:03:36 AM EDT
4/3/2025, 12:03:36 PM UTC
Commit: 461ca8d8fe5b1efd4c01fc87e5b5eb592e2d154a
XML generation date: 2025-03-07 13:15:03.044
Product last modified at: 2025-03-08T08:22:42.307Z
1% for the planet logo
PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

DNAM-1/CD226 (E8L9G) XP® Rabbit mAb #66631

Filter:
  • WB
  • IHC
Western Blotting Image 1: DNAM-1/CD226 (E8L9G) XP® Rabbit mAb
Western blot analysis of extracts from various cell lines using DNAM-1/CD226 (E8L9G) XP® Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).

To Purchase # 66631

Supporting Data

REACTIVITY H
SENSITIVITY Endogenous
MW (kDa) 70
Source/Isotype Rabbit IgG
Application Key:
  • WB-Western Blotting 
  • IHC-Immunohistochemistry 
Species Cross-Reactivity Key:
  • H-Human 
  • Related Products

Product Information

Product Usage Information

Application Dilution
Western Blotting 1:1000
IHC Leica Bond 1:50 - 1:200
Immunohistochemistry (Paraffin) 1:50 - 1:200

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

For a carrier free (BSA and azide free) version of this product see product #66991.

Protocol

Specificity / Sensitivity

DNAM-1/CD226 (E8L9G) XP® Rabbit mAb recognizes endogenous levels of total DNAM-1/CD226 protein.

Species Reactivity:

Human

Source / Purification

Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of human DNAM-1/CD226 protein.

Background

DNAM-1/CD226 is a member of the immunoglobulin-like superfamily. It is expressed on T cells, natural killer (NK) cells, monocytes, and macrophages. It is a major activating receptor on NK cells. Engagement with its ligands Nectin-2 (CD112) and PVR (poliovirus receptor, also known as CD155) on the target cells leads to downstream activation of ERK, AKT, and PLCg2 signaling pathways that are crucial for NK activation and NK-mediated killing (1-4). LFA-1 physically associates with DNAM-1/CD226 and contributes to its signaling (5). Activating signal of DNAM-1/CD226 is counterbalanced by inhibitory receptors TIGIT and CD96, competing for the common ligands CD122 and PVR (6,7).
For Research Use Only. Not For Use In Diagnostic Procedures.
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