R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
DIS3 (E5I1Y) Rabbit mAb #84348
Filter:
- WB
- IP
Supporting Data
| REACTIVITY | H Mk |
| SENSITIVITY | Endogenous |
| MW (kDa) | 120 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
- Mk-Monkey
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:100 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
DIS3 (E5I1Y) Rabbit mAb recognizes endogenous levels of total DIS3 protein.
Species Reactivity:
Human, Monkey
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val928 of human DIS3 protein.
Background
DIS3, also known as RRP44, is a conserved protein responsible for the core catalytic activities of the exosome complex, which degrades RNA (1-3). With two catalytic sites, DIS3 possesses both endoribonucleolytic and 3’-to-5’ exoribonucleolytic activity (2,4). It plays very diverse roles in RNA metabolism, including control of aberrant pre-mRNA turnover, gene expression, and RNA processing (5-9). DIS3 also plays a role in mitotic progression by helping form kinetochores (9,10). Additionally, DIS3 is implicated in antibody diversity by helping the exosome target activation-induced cytidine deaminase to the DNA strands in B cells. DIS3 and other exosome components are upregulated during V(D)J recombination and B cells in knockout mice are unable to develop past the pro-B cell stage (11,12). Mutations in the DIS3 gene have been well described in multiple myeloma (13,14).
- Vincent, H.A. and Deutscher, M.P. (2006) J Biol Chem 281, 29769-75.
- Dziembowski, A. et al. (2007) Nat Struct Mol Biol 14, 15-22.
- Liu, Q. et al. (2006) Cell 127, 1223-37.
- Lebreton, A. et al. (2008) Nature 456, 993-6.
- Bousquet-Antonelli, C. et al. (2000) Cell 102, 765-75.
- Milligan, L. et al. (2005) Mol Cell Biol 25, 9996-10004.
- Mukherjee, D. et al. (2002) EMBO J 21, 165-74.
- Allmang, C. et al. (1999) EMBO J 18, 5399-410.
- Murakami, H. et al. (2007) PLoS One 2, e317.
- Kinoshita, N. et al. (1991) Mol Cell Biol 11, 5839-47.
- Basu, U. et al. (2011) Cell 144, 353-63.
- Laffleur, B. et al. (2022) Sci Immunol 7, eabn2738.
- Chapman, M.A. et al. (2011) Nature 471, 467-72.
- Walker, B.A. et al. (2012) Blood 120, 1077-86.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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