Render Target: SSR
Render Timestamp: 2024-11-27T18:05:01.810Z
Commit: d79925545b26f8827f92d145dadc6f0527debdb1
XML generation date: 2024-08-01 15:29:56.456
Product last modified at: 2024-07-11T11:45:07.984Z
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PDP - Template Name: Polyclonal Antibody
PDP - Template ID: *******59c6464

DIS3 Antibody #20035

Filter:
  • WB

    Supporting Data

    REACTIVITY H M R Mk
    SENSITIVITY Endogenous
    MW (kDa) 120
    SOURCE Rabbit
    Application Key:
    • WB-Western Blotting 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • R-Rat 
    • Mk-Monkey 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    DIS3 Antibody recognizes endogenous levels of total DIS3 protein. This antibody detects an 85 kDa band of unknown origin.

    Species Reactivity:

    Human, Mouse, Rat, Monkey

    Source / Purification

    Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val928 of human DIS3 protein. Antibodies are purified by peptide affinity chromatography.

    Background

    DIS3, also known as RRP44, is a conserved protein responsible for the core catalytic activities of the exosome complex, which degrades RNA (1-3). With two catalytic sites, DIS3 possesses both endoribonucleolytic and 3’-to-5’ exoribonucleolytic activity (2,4). It plays very diverse roles in RNA metabolism, including control of aberrant pre-mRNA turnover, gene expression, and RNA processing (5-9). DIS3 also plays a role in mitotic progression by helping form kinetochores (9,10). Additionally, DIS3 is implicated in antibody diversity by helping the exosome target activation-induced cytidine deaminase to the DNA strands in B cells. DIS3 and other exosome components are upregulated during V(D)J recombination and B cells in knockout mice are unable to develop past the pro-B cell stage (11,12). Mutations in the DIS3 gene have been well described in multiple myeloma (13,14).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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