R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
DFCP1 (E9Q1S) Rabbit mAb #38419
Filter:
- WB
- IP
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | 82 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:100 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
DFCP1 (E9W1S) Rabbit mAb recognizes endogenous levels of total DFCP1 protein.
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro336 of human DFCP1 protein.
Background
Double FYVE-containing protein 1 (DFCP1; gene name ZFYVE1), which was identified from a human bone marrow library, contains two carboxyl terminal FYVE domains that function as binding sites for phosphatidylinositol 3-phosphate (PI3P) (1). PI3P generated predominantly by the class III PI3-kinase VPS34 plays a key role in membrane trafficking as well as autophagy (2,3). DFCP1 is primarily localized to the Golgi and endoplasmic reticulum (ER) (4,5). However, during autophagy DFCP1 re-localizes to subdomains of the ER, the omegasome, which become the sites for autophagosome formation (6,7).
- Derubeis, A.R. et al. (2000) Gene 255, 195-203.
- Corvera, S. (2001) Traffic 2, 859-66.
- Yan, Y. and Backer, J.M. (2007) Biochem Soc Trans 35, 239-41.
- Ridley, S.H. et al. (2001) J Cell Sci 114, 3991-4000.
- Cheung, P.C. et al. (2001) Biochem J 355, 113-21.
- Itakura, E. and Mizushima, N. (2010) Autophagy 6, 764-76.
- McAlpine, F. et al. (2013) Autophagy 9, 361-73.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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