Dectin-1/Clec7a Antibody #23042
Filter:
- WB
Western blot analysis of extracts from SIM-A9 and Neuro-2a cells, untreated (-) or treated with peptide N-glycosidase F (PNGase F; +), using Dectin-1/Clec7a Antibody (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).
Supporting Data
| REACTIVITY | M |
| SENSITIVITY | Endogenous |
| MW (kDa) | 10, 22 |
| SOURCE | Rabbit |
Application Key:
- WB-Western Blotting
Species Cross-Reactivity Key:
- M-Mouse
- Related Products
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, and 50% glycerol. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
Dectin-1/Clec7a Antibody recognizes endogenous levels of total Dectin-1 protein.
Species Reactivity:
Mouse
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding His26 of mouse Dectin-1 protein. Antibodies are purified by peptide affinity chromatography.
Background
Dectin-1, also known as Clec7a, is a C-type lectin receptor expressed by macrophages, monocytes, dendritic cells, neutrophils, and a subset of γδ T cells (1,2). Dectin-1 is a glycoprotein with eight different isoforms, generated through alternative splicing (3-5). It plays an important role in anti-fungal immunity by acting as a pattern recognition receptor for β-glucans found on the cell wall of fungi and some bacteria (5,6). Dectin-1 is composed of a short amino-terminal cytoplasmic domain containing an ITAM-like motif, a transmembrane domain, and an extracellular carboxy-terminal C-type lectin domain (5). Dectin-1 recognizes β-glucans through its C-type lectin domain and transduces signals through its ITAM-like motif by recruiting and activating Syk (7,8). Dendritic cells activated through Dectin-1 promote differentiation of Th17 cells by producing IL-6 and IL-23 (9).
Dectin-1 may also play a role in neurodegenerative diseases. CLEC7A, the gene that encodes Dectin-1, is upregulated in microglia, the resident macrophages of the brain, in mouse neurodegenerative disease models, including Alzheimer’s disease (10).
Dectin-1 may also play a role in neurodegenerative diseases. CLEC7A, the gene that encodes Dectin-1, is upregulated in microglia, the resident macrophages of the brain, in mouse neurodegenerative disease models, including Alzheimer’s disease (10).
- Taylor, P.R. et al. (2002) J Immunol 169, 3876-82.
- Martin, B. et al. (2009) Immunity 31, 321-30.
- Ariizumi, K. et al. (2000) J Biol Chem 275, 20157-67.
- Kato, Y. et al. (2006) Biol Pharm Bull 29, 1580-6.
- Willment, J.A. et al. (2001) J Biol Chem 276, 43818-23.
- Brown, G.D. and Gordon, S. (2001) Nature 413, 36-7.
- Rogers, N.C. et al. (2005) Immunity 22, 507-17.
- Underhill, D.M. et al. (2005) Blood 106, 2543-50.
- LeibundGut-Landmann, S. et al. (2007) Nat Immunol 8, 630-8.
- Keren-Shaul, H. et al. (2017) Cell 169, 1276-1290.e17.
限制使用
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