Render Target: SSR
Render Timestamp: 2024-11-14T22:43:19.040Z
Commit: 3c1f305a63297e594ac8d7bb5424007d592d68be
XML generation date: 2024-09-30 01:55:52.941
Product last modified at: 2024-09-30T08:01:12.310Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77

DDX4 (2F9H5) Mouse mAb #5555

Filter:
  • WB
  • IP

    Supporting Data

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa) 80
    Source/Isotype Mouse IgG1
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:50

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    DDX4 (2F9H5) Mouse mAb detects endogenous levels of total DDX4 protein.

    Species Reactivity:

    Human

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a purified recombinant fragment of human DDX4.

    Background

    DDX4 is an ATP-dependent DEAD-box RNA helicase found in the chromatoid body of the germ cells (1). This enzyme is specific to germ cells and is necessary for germ cell development (2). Mouse DDX4 was shown to interact with Dicer, suggesting a role in microRNA-mediated RNA silencing (1).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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