R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
DcR2 (E5X3W) Rabbit mAb #51558
Filter:
- WB
- IP
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | 45-60 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:200 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
DcR2 (E5X3W) Rabbit mAb recognizes endogenous levels of total DcR2 protein. A band of unknown origin is detected at around 22 kDa in some cell lines.
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu285 of human DcR2 protein.
Background
The tumor necrosis factor receptor family, which includes TNF-RI, Fas, DR3, DR4, DR5, and DR6, plays an important role in the regulation of apoptosis in various physiological systems (1,2). The receptors are activated by a family of cytokines that include TNF, FasL, and TNF-related apoptosis-inducing ligand (TRAIL). They are characterized by a highly conserved extracellular region containing cysteine-rich repeats and a conserved intracellular region of about 80 amino acids termed the death domain (DD). The DD is important for transducing the death signal by recruiting other DD containing adaptor proteins (FADD, TRADD, RIP) to the death-inducing signaling complex (DISC), resulting in activation of caspases.
Death receptor signaling can be controlled by a family of decoy receptors (DcR1, DcR2, and DcR3) that lack a cytoplasmic DD and inhibit death receptor-mediated apoptosis by competing for ligand binding (3-5). Expression of decoy receptors can contribute to chemosensitivity and may provide a mechanism for regulation of apoptosis in certain types of cancer (6-8).
Death receptor signaling can be controlled by a family of decoy receptors (DcR1, DcR2, and DcR3) that lack a cytoplasmic DD and inhibit death receptor-mediated apoptosis by competing for ligand binding (3-5). Expression of decoy receptors can contribute to chemosensitivity and may provide a mechanism for regulation of apoptosis in certain types of cancer (6-8).
- Nagata, S. (1997) Cell 88, 355-65.
- Thorburn, A. (2004) Cell Signal 16, 139-44.
- Sheridan, J.P. et al. (1997) Science 277, 818-21.
- Marsters, S.A. et al. (1997) Curr Biol 7, 1003-6.
- Pitti, R.M. et al. (1998) Nature 396, 699-703.
- Liu, X. et al. (2005) Cancer Res 65, 9169-75.
- Spalding, A.C. et al. (2002) Oncogene 21, 260-71.
- Bernard, D. et al. (2001) J Biol Chem 276, 27322-8.
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