R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
DcR1 (E9Z4U) Rabbit mAb #19548
Filter:
- WB
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | 62 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
DcR1 (E9Z4U) Rabbit mAb recognizes endogenous levels of total DcR1 protein.
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala223 of human DcR1 protein.
Background
The tumor necrosis factor receptor family, which includes TNF-RI, Fas, DR3, DR4, DR5, and DR6, plays an important role in the regulation of apoptosis in various physiological systems (1,2). The receptors are activated by a family of cytokines that include TNF, FasL, and TNF-related apoptosis-inducing ligand (TRAIL). They are characterized by a highly conserved extracellular region containing cysteine-rich repeats and a conserved intracellular region of about 80 amino acids termed the death domain (DD). The DD is important for transducing the death signal by recruiting other DD containing adaptor proteins (FADD, TRADD, RIP) to the death-inducing signaling complex (DISC), resulting in activation of caspases.
Death receptor signaling is also controlled by a family of decoy receptors (DcR1, DcR2, and DcR3) which lack a cytoplasmic DD and inhibit death receptor-mediated apoptosis by competing for ligand (3-5). Expression of decoy receptors provide a mechanism for certain types of cancer to regulate apoptosis and can contribute to chemosensitivity (6-8).
Death receptor signaling is also controlled by a family of decoy receptors (DcR1, DcR2, and DcR3) which lack a cytoplasmic DD and inhibit death receptor-mediated apoptosis by competing for ligand (3-5). Expression of decoy receptors provide a mechanism for certain types of cancer to regulate apoptosis and can contribute to chemosensitivity (6-8).
- Nagata, S. (1997) Cell 88, 355-65.
- Thorburn, A. (2004) Cell Signal 16, 139-44.
- Sheridan, J.P. et al. (1997) Science 277, 818-21.
- Marsters, S.A. et al. (1997) Curr Biol 7, 1003-6.
- Pitti, R.M. et al. (1998) Nature 396, 699-703.
- Liu, X. et al. (2005) Cancer Res 65, 9169-75.
- Spalding, A.C. et al. (2002) Oncogene 21, 260-71.
- Bernard, D. et al. (2001) J Biol Chem 276, 27322-8.
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For Research Use Only. Not For Use In Diagnostic Procedures.
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