R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
DCLK1/DCAMKL1 (D2U3L) XP® Rabbit mAb (BSA and Azide Free) #10208
Filter:
- WB
- IHC
Supporting Data
| REACTIVITY | H M R |
| SENSITIVITY | Endogenous |
| MW (kDa) | 82 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IHC-Immunohistochemistry
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
Product Information
Product Usage Information
This product is the carrier free version of product #62257. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol.
This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us. Optimal dilutions/concentrations should be determined by the end user.
BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity.
This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us. Optimal dilutions/concentrations should be determined by the end user.
BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity.
Formulation
Supplied in 1X PBS (10 mM Na2HPO4, 3 mM KCl, 2 mM KH2PO4, and 140 mM NaCl (pH 7.8)). BSA and Azide Free.
For standard formulation of this product see product #62257
For standard formulation of this product see product #62257
Storage
Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance.
Specificity / Sensitivity
DCLK1/DCAMKL1 (D2U3L) XP® Rabbit mAb recognizes endogenous levels of total DCLK1/DCAMKL1 protein. The identity of the faint lower molecular weight bands is not known.
Species Reactivity:
Human, Mouse, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human DCLK1/DCAMKL1 protein.
Background
Doublecortin-like kinase 1 (DCLK1, DCAMKL1) is a serine/threonine kinase that belongs to the CaM kinase family and shares homology with the neuronal microtubule binding protein doublecortin. DCLK1 is thought to be involved in calcium signaling pathways controlling neuronal development in the embryonic brain (1,2). The kinase also functions in the mature nervous system and is highly expressed in regions of active neurogenesis in the neocortex and cerebellum (3,4). Research studies suggest that the DCLK1 kinase is highly expressed in subpopulations of cells within the colon and gastric epithelium and in the pancreas (5-8). The nature of these cell populations, whether normal, stem-like, or tumor-initiating, is unclear.
- Burgess, H.A. et al. (1999) J Neurosci Res 58, 567-75.
- Tanaka, T. et al. (2006) Cereb Cortex 16 Suppl 1, i69-73.
- Shin, E. et al. (2013) Nat Commun 4, 1440.
- Saaltink, D.J. et al. (2012) J Comp Neurol 520, 2805-23.
- May, R. et al. (2009) Stem Cells 27, 2571-9.
- May, R. et al. (2010) Am J Physiol Gastrointest Liver Physiol 299, G303-10.
- Saqui-Salces, M. et al. (2011) Histochem Cell Biol 136, 191-204.
- Qu, D. et al. (2014) Am J Physiol Gastrointest Liver Physiol 306, G404-11.
限制使用
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专品专有“专供研究使用”的专专或专似的专专声明， 且未专得美国食品和专品管理局或其他外国或国内专管机专专专任何用途的批准、准专或专可。客专不得将任何专品用于任何专断或治专目的， 或以任何不符合专专声明的方式使用专品。CST 专售或专可的专品提供专作专最专用专的客专，且专用于研专用途。将专品用于专断、专防或治专目的， 或专专售（专独或作专专成）或其他商专目的而专专专品，均需要 CST 的专独专可。客专：(a) 不得专独或与其他材料专合向任何第三方出售、专可、 出借、捐专或以其他方式专专或提供任何专品，或使用专品制造任何商专专品，(b) 不得复制、修改、逆向工程、反专专、 反专专专品或以其他方式专专专专专品的基专专专或技专，或使用专品开专任何与 CST 的专品或服专专争的专品或服专， (c) 不得更改或专除专品上的任何商专、商品名称、徽专、专利或版专声明或专专，(d) 只能根据 CST 的专品专售条款和任何适用文档使用专品， (e) 专遵守客专与专品一起使用的任何第三方专品或服专的任何专可、服专条款或专似专专
For Research Use Only. Not For Use In Diagnostic Procedures.
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