R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
DCAF16 (F8E1P) Rabbit mAb #52485
Filter:
- WB
- IP
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | 25 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
DCAF16 (F8E1P) Rabbit mAb recognizes endogenous levels of total DCAF16 protein. This antibody may cross-react with proteins of unknown origin around 50 kDa and 100 kDa.
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human DCAF16 protein.
Background
The CUL4-DDB1 ubiquitin ligase complex mediates the degradation of target proteins involved in various cellular processes, including DNA damage repair, cell cycle progression, transcription, and development (1-5). DDB1- and CUL4-associated factor 16 (DCAF16) is an E3 ubiquitin ligase that, like other members of the DCAF family, functions as a substrate receptor to recruit the CUL4-DDB1 complex to target proteins for ubiquitination (1,6). DCAF16 is often studied in the context of targeted protein degradation (TPD), where it is utilized to specifically mark disease-associated nuclear proteins for ubiquitination and removal (7). For instance, DCAF16 was found to mediate degradation of FKBP12 and BRD4 protein following treatment with heterobifunctional degrader compounds (6).
- Lee, J. and Zhou, P. (2007) Mol Cell 26, 775-80.
- Higa, L.A. and Zhang, H. (2007) Cell Div 2, 5.
- Ghosh, P. et al. (2008) Cell Cycle 7, 373-81.
- Ohtake, F. et al. (2007) Nature 446, 562-6.
- Shiyanov, P. et al. (1999) J Biol Chem 274, 35309-12.
- Zhang, X. et al. (2019) Nat Chem Biol 15, 737-746.
- Békés, M. et al. (2022) Nat Rev Drug Discov 21, 181-200.
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For Research Use Only. Not For Use In Diagnostic Procedures.
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