DAZ1 Antibody #12706
Filter:
- WB
- IP
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | 82 |
| SOURCE | Rabbit |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:100 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
DAZ1 Antibody recognizes endogenous levels of total DAZ1 protein.
Species Reactivity:
Human
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro311 of human DAZ1 protein. Antibodies are purified by protein A and peptide affinity chromatography.
Background
The human Deleted in AZoospermia (DAZ) genes encode a family of RNA-binding proteins that are expressed exclusively in the germ cells and play a role in the regulation of mRNA translation (1). Members of the family include DAZ, DAZL, and BOULE. The essential role of DAZL and BOULE in spermatogenesis has been established (2-4), but the role of DAZ remains unclear. Men with deletions in the DAZ1 gene can produce sperm, although in lower numbers, and are capable of reproduction and transmission of the genetic lesions (5-7).
- Yen, P.H. (2004) Int J Androl 27, 125-9.
- Ruggiu, M. et al. (1997) Nature 389, 73-7.
- Houston, D.W. and King, M.L. (2000) Development 127, 447-56.
- Karashima, T. et al. (2000) Development 127, 1069-79.
- Calogero, A.E. et al. (2002) J Endocrinol Invest 25, 631-4.
- Kühnert, B. et al. (2004) Hum Reprod 19, 886-8.
- Reynolds, N. and Cooke, H.J. (2005) Reprod Biomed Online 10, 72-80.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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