Daxx Antibody #4532
Inquiry Info. # 4532
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Supporting Data
| REACTIVITY | H M R |
| SENSITIVITY | Endogenous |
| MW (kDa) | 120 |
| SOURCE | Rabbit |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:100 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
Daxx Antibody detects endogenous levels of total Daxx protein.
Species Reactivity:
Human, Mouse, Rat
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to a region around residue 260 of Daxx. Antibodies are purified by protein A and peptide affinity chromatography.
Background
Daxx is a ubiquitously expressed protein that was originally identified through a yeast two-hybrid screen as an interactor with the cytoplasmic domain of Fas. It was found to enhance Fas-mediated apoptosis and activate the JNK pathway (1). However, additional studies have revealed that Daxx is actually a nuclear protein localizing to promyelocytic leukemia oncogenic domains (PODs) (2,3). Nuclear interactions have since been observed with CENP-C (4), Pax3 (5), DNA methyltransferase I (6) and chromatin-associated proteins, including histone deacetylase II, H2A, H2B, H3, H4 and Dek (5). Roles for Daxx have been suggested in transcriptional repression and cell cycle control. Loss of Daxx in mice leads to embryonic lethality with extensive developmental apoptosis, suggesting a role for Daxx directly or indirectly in suppressing cell death (5). Furthermore, inhibition of Daxx expression using RNAi has confirmed Daxx to be anti-apoptotic and to repress transcriptional activity of targets including NF-κB and E2F-1 (7).
- Yang, X. et al. (1997) Cell 89, 1067-76.
- Torii, S. et al. (1999) EMBO J 18, 6037-49.
- Li, H. et al. (2000) Mol Cell Biol 20, 1784-96.
- Pluta, A.F. et al. (1998) J Cell Sci 111 ( Pt 14), 2029-41.
- Hollenbach, A.D. et al. (1999) EMBO J 18, 3702-11.
- Michaelson, J.S. et al. (1999) Genes Dev 13, 1918-23.
- Michaelson, J.S. and Leder, P. (2003) J Cell Sci 116, 345-52.
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For Research Use Only. Not For Use In Diagnostic Procedures.
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