R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
DAX1 (D2F1) Rabbit mAb #13538
Filter:
- WB
- IP
- IHC
- IF
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | 48 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
- IHC-Immunohistochemistry
- IF-Immunofluorescence
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:100 |
| Immunohistochemistry (Paraffin) | 1:400 |
| Immunofluorescence (Immunocytochemistry) | 1:3200 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
DAX1 (D2F1) recognizes endogenous levels of total DAX1 protein.
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly330 of human DAX1 protein.
Background
DSS-AHC critical region on the X chromosome protein 1 (DAX1) is an orphan nuclear receptor encoded by the nuclear receptor subfamily 0 group B member 1 (NR0B1) gene. DAX1 possesses an atypical DNA binding domain that allows it to form heterodimeric complexes with DNA binding partners and repress transcriptional activity (1,2). During development, DAX1 is important for establishment of the hypothalamic-pituitary-adrenal gonadal axis. The receptor is essential for development of several important hormone-producing organs that determine this axis, including the adrenal glands, pituitary, hypothalamus, and the male and female reproductive organs (3,4). Research studies suggest that DAX1 plays a role in maintenance of pluripotency in embryonic stem cells (5,6). Loss of DAX1 function through deletion or mutation results in adrenal insufficiency and hypogonadotropic hypogonadism (7), while duplication of the NR0B1 gene on the X-chromosome causes dosage-sensitive sex reversal (8).
- Iyer, A.K. et al. (2006) Mol Endocrinol 20, 2326-42.
- Iyer, A.K. and McCabe, E.R. (2004) Mol Genet Metab 83, 60-73.
- Niakan, K.K. and McCabe, E.R. (2005) Mol Genet Metab 86, 70-83.
- McCabe, E.R. (2007) Mol Cell Endocrinol 265-266, 179-82.
- Uranishi, K. et al. (2013) Mol Cell Biol 33, 2056-66.
- Wang, Q. and Cooney, A.J. (2013) Adv Exp Med Biol 786, 287-306.
- Jadhav, U. et al. (2011) Mol Cell Endocrinol 346, 65-73.
- Sanlaville, D. et al. (2004) Am J Med Genet A 128A, 325-30.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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