Cytochrome b Antibody #69202
Filter:
- WB
- IP
Supporting Data
| REACTIVITY | H Mk |
| SENSITIVITY | Endogenous |
| MW (kDa) | 26 |
| SOURCE | Rabbit |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
- Mk-Monkey
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, and 50% glycerol. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
Cytochrome b Antibody recognizes endogenous levels of total cytochrome b protein.
Species Reactivity:
Human, Monkey
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro208 of human cytochrome b protein. Antibodies are purified by peptide affinity chromatography.
Background
Cytochrome b, encoded by the gene MT-CYB, is a subunit of ubiquinol-cytochrome c reductase, also known as cytochrome bc1, or complex III, of the mitochondrial respiratory (electron transport) chain (1). This protein complex mediates electron transfer from ubiquinol to cytochrome c, contributing to the generation of a proton gradient that powers ATP synthesis (2). Cytochrome b is an integral membrane protein and notable for being the only protein within complex III that is encoded by the mitochondrial genome. Genetic sequence analyses have identified mutations in MT-CYB that are linked to a variety of diseases and pathologies (3,4). Natural variations in the sequence of MT-CYB have also proven highly useful as a tool to determine phylogenetic relationships between organisms (5).
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For Research Use Only. Not For Use In Diagnostic Procedures.
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