R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
Cyclin B1 (F7U1O) Rabbit mAb #61976
Filter:
- WB
- IP
- IHC
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | 55 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
- IHC-Immunohistochemistry
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Simple Western™ | 1:50 - 1:250 |
| Immunoprecipitation | 1:100 |
| Immunohistochemistry (Paraffin) | 1:50 - 1:200 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
For a carrier free (BSA and azide free) version of this product see product #67873.
For a carrier free (BSA and azide free) version of this product see product #67873.
Protocol
Specificity / Sensitivity
Cyclin B1 (F7U1O) Rabbit mAb recognizes endogenous levels of total cyclin B1 protein. Non-specific staining was observed in cardiac and skeletal muscle by immunohistochemistry.
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asn275 of human cyclin B1 protein.
Background
Cyclins are a family of proteins that activate specific cyclin-dependent kinases required for progression through the cell cycle. The entry of all eukaryotic cells into mitosis is regulated by activation of cdc2/cdk1 at the G2/M transition. This activation is a multi-step process that begins with the binding of the regulatory subunit, cyclin B1, to cdc2/cdk1 to form the mitosis-promoting factor (MPF). MPF remains in the inactive state until phosphorylation of cdc2/cdk1 at Thr161 by cdk activating kinase (CAK) (1,2) and dephosphorylation of cdc2/cdk1 at Thr14/Tyr15 by cdc25C (3-5). Five cyclin B1 phosphorylation sites (Ser116, 126, 128, 133, and 147) are located in the cytoplasmic retention signal (CRS) domain and are thought to regulate the translocation of cyclin B1 to the nucleus at the G2/M checkpoint, promoting nuclear accumulation and initiation of mitosis (6-9). While MPF itself can phosphorylate Ser126 and Ser128, polo-like kinase 1 (PLK1) phosphorylates cyclin B1 preferentially at Ser133 and possibly at Ser147 (6,10). At the end of mitosis, cyclin B1 is targeted for degradation by the anaphase-promoting complex (APC), allowing for cell cycle progression (11). Research studies have shown that cyclin B1 is overexpressed in breast, prostate, and non-small cell lung cancers (12-14).
- Lorca, T. et al. (1992) EMBO J 11, 2381-90.
- Harper, J.W. and Elledge, S.J. (1998) Genes Dev 12, 285-9.
- Norbury, C. et al. (1991) EMBO J 10, 3321-9.
- McGowan, C.H. and Russell, P. (1993) EMBO J 12, 75-85.
- Atherton-Fessler, S. et al. (1994) Mol Biol Cell 5, 989-1001.
- Toyoshima-Morimoto, F. et al. (2001) Nature 410, 215-20.
- Li, J. et al. (1997) Proc Natl Acad Sci U S A 94, 502-7.
- Takizawa, C.G. and Morgan, D.O. (2000) Curr Opin Cell Biol 12, 658-65.
- Santos, S.D. et al. (2012) Cell 149, 1500-13.
- Jackman, M. et al. (2003) Nat Cell Biol 5, 143-8.
- Gong, D. and Ferrell, J.E. (2010) Mol Biol Cell 21, 3149-61.
- Mashal, R.D. et al. (1996) Cancer Res 56, 4159-63.
- Kawamoto, H. et al. (1997) Am J Pathol 150, 15-23.
- Soria, J.C. et al. (2000) Cancer Res 60, 4000-4.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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