Name: CXXC1 (D1R5R) Rabbit mAb
Price: 327.00 USD
Availability: InStock

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IP
ChIP
C&R
C&T

Supporting Data

REACTIVITY	H
SENSITIVITY	Endogenous
MW (kDa)	82
Source/Isotype	Rabbit IgG

Application Key:

WB-Western Blotting
IP-Immunoprecipitation
ChIP-Chromatin Immunoprecipitation
C&R-CUT & RUN
C&T-CUT & Tag

Species Cross-Reactivity Key:

H-Human

Product Information

Product Usage Information

For optimal ChIP results, use 5 μl of antibody and 10 μg of chromatin (approximately 4 x 10⁶ cells) per IP. This antibody has been validated using SimpleChIP^® Enzymatic Chromatin IP Kits.

The CUT&RUN dilution was determined using CUT&RUN Assay Kit #86652.

The CUT&Tag dilution was determined using CUT&Tag Assay Kit #77552.

Application	Dilution
Western Blotting	1:1000
Immunoprecipitation	1:100
Chromatin IP	1:100
CUT&RUN	1:100
CUT&Tag	1:100

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

CXXC1 (D1R5R) Rabbit mAb recognizes endogenous levels of total CXXC1 protein. In Western blot analysis, the antibody detects a 32kDa protein of unknown identity.

Species Reactivity:

Human

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly107 of human CXXC1 protein.

Background

The CXXC finger protein 1 (CXXC1, CGBP, CFP1) is a key subunit of the human SET1 histone methyltransferase complex (1,2) that methylates histone H3 at Lys4 to create a mark of transcriptionally active promoters (3,4). CXXC1 is enriched at CpG islands where it selectively binds non-methylated CpG motifs to provide a link between global H3K4 methylation and CpG islands (5). Research studies have revealed a role for CXXC1 in the maintenance of cytosine methylation through direct interaction with DNMT1 (6-9). The epigenetic functions of CXXC1 are critical for normal embryonic development. Targeted deletion of the murine Cxxc1 gene results in early embryonic lethality while Cxxc1- embryonic stem (ES) cells exhibit increased apoptosis and fail to undergo differentiation in vitro following withdrawal of leukemia inhibitory factor LIF (6).

Database Links

UniProt ID: Q9P0U4

Entrez-Gene Id: 30827

限制使用

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For Research Use Only. Not For Use In Diagnostic Procedures.

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CXXC1 (D1R5R) Rabbit mAb #40672