R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
CXCL9/MIG (E6Z5W) Rabbit mAb (BSA and Azide Free) #37438
Filter:
- WB
- IHC
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | 15 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IHC-Immunohistochemistry
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
This product is the carrier-free version of product #30327. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol.
This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier-free formulation for chromatin profiling, please contact us. Optimal dilutions/concentrations should be determined by the end user.
BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity.
This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier-free formulation for chromatin profiling, please contact us. Optimal dilutions/concentrations should be determined by the end user.
BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity.
Formulation
Supplied in 1X PBS (10 mM Na2HPO4, 3 mM KCl, 2 mM KH2PO4, and 140 mM NaCl (pH 7.8)). BSA and Azide Free.
For standard formulation of this product see product #30327.
Storage
Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance.
Specificity / Sensitivity
CXCL9/MIG (E6Z5W) Rabbit mAb (BSA and Azide Free) recognizes endogenous levels of total CXCL9/MIG protein. This antibody does not cross-react with CXCL10, CXCL11, or CXCL2 protein.
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with recombinant protein specific to full-length human CXCL9/MIG protein.
Background
C-X-C motif chemokine ligand 9 (CXCL9, MIG) is a soluble chemokine expressed by multiple cell types, such as endothelial cells, monocytes, and tumor cells. The expression of CXCL9 is upregulated in response to proinflammatory cytokines, such as IFN-γ and TNF-α. (1,2). CXCL9 binds to CXCR3, a GPCR that is expressed on the surface of multiple populations of immune cells, particularly exclusively activated T lymphocytes (3). Binding of CXCL9 to CXCR3 promotes T cell chemotaxis and infiltration into sites of inflammation that form in settings such as viral infection and tumorigenesis (4-6). Research studies have demonstrated production of CXCL9 by tumor cells mediates tumor suppression through the recruitment of tumor antigen-specific T cells (7).
- Shin, S.Y. et al. (2010) J Biol Chem 285, 30731-40.
- Hiroi, M. and Ohmori, Y. (2003) Biochem J 376, 393-402.
- Loetscher, M. et al. (1996) J Exp Med 184, 963-9.
- Tensen, C.P. et al. (1999) J Invest Dermatol 112, 716-22.
- Han, K. et al. (2020) Am J Respir Cell Mol Biol, doi: 10.1165/rcmb.2020-0354OC.
- Russo, E. et al. (2020) J Leukoc Biol 108, 673-685.
- Gorbachev, A.V. et al. (2007) J Immunol 178, 2278-86.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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