CUTL1 Antibody #81557
Filter:
- WB
- IP
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | 200 |
| SOURCE | Rabbit |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:200 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
CUTL1 Antibody recognizes endogenous levels of total CUTL1 protein. It recognizes the p200 isoform (the full-length) and is not expected to recognize the p110 and p75 isoforms.
Species Reactivity:
Human
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gln639 of human CUTL1 protein. Antibodies are purified by protein A and peptide affinity chromatography.
Background
CUTL1 (Cut-like 1), also known as CUX1 (Cut homeobox 1) (CUX1), is a transcription factor that has been implicated in many cellular processes in different tissues, such as cell migration, neuronal differentiation, and DNA repair (1-5). CUTL1 expression and activities are altered in cancer. Research studies have shown the CUTL1 gene to be a frequent target of loss-of-heterozygocity in various cancers (6,7). On the other hand, CUTL1 expression is elevated in many cancers and is associated with shorter disease-free survival (8). These accumulating evidence suggest that decreased CUTL1 expression promote tumor initiation and increased CUTL1 expression facilitates tumor progression (9). While full-length CUTL1 is about 200 kDa (p200), short forms p110 and p75 can also be generated by proteolytic processing and alternative transcription initiation site, respectively (10, 11).
- Rodríguez-Tornos, F.M. et al. (2016) Neuron 89, 494-506.
- Ramdzan, Z.M. et al. (2015) Oncotarget 6, 3613-26.
- Ramdzan, Z.M. et al. (2014) PLoS Biol 12, e1001807.
- Kedinger, V. et al. (2009) J Biol Chem 284, 27701-11.
- Vadnais, C. et al. (2012) Nucleic Acids Res 40, 4483-95.
- McNerney, M.E. et al. (2013) Blood 121, 975-83.
- Ramdzan, Z.M. and Nepveu, A. (2014) Nat Rev Cancer 14, 673-82.
- Michl, P. et al. (2005) Cancer Cell 7, 521-32.
- Hulea, L. and Nepveu, A. (2012) Gene 497, 18-26.
- Moon, N.S. et al. (2001) Mol Cell Biol 21, 6332-45.
- Goulet, B. et al. (2002) Cancer Res 62, 6625-33.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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