渲染靶标:SSR
Render Timestamp: 2025-03-17T22:10:53.738Z
Commit: 9fc0f116116d9da247dc8ddd4e5fe811153412e1
XML generation date: 2024-08-01 15:23:29.425
Product last modified at: 2025-02-28T14:30:10.904Z
1% for the Planet 标识
PDP - Template Name: Polyclonal Antibody
PDP - Template ID: *******59c6464

CUL1 Antibody #4995

Filter:
  • WB
  • IP
Western Blotting Image 1: CUL1 Antibody
Western blot analysis of extracts from K-562, untreated or MLN4924-treated (0.3uM, 16 hours) probed with CUL1 (left), #2754 NEDD8 (19E3) Rabbit mAb (middle), or #8457 beta-Actin (D6A8) Rabbit mAb (right).

To Purchase # 4995

Cat. #

Size

Price

Inventory

4995S
100 µl
¥4,550

库存充足

Supporting Data

REACTIVITY H M R
SENSITIVITY Endogenous
MW (kDa) 90
SOURCE Rabbit
Application Key:
  • WB-Western Blotting 
  • IP-Immunoprecipitation 
Species Cross-Reactivity Key:
  • H-Human 
  • M-Mouse 
  • R-Rat 
  • Related Products

Product Information

Product Usage Information

Application Dilution
Western Blotting 1:1000
Immunoprecipitation 1:50

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

The CUL1 Antibody detects endogenous levels of CUL1 protein.

Species Reactivity:

Human, Mouse, Rat

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues of human CUL1. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Ubiquitin can be covalently linked to many cellular proteins by the ubiquitination process, which targets proteins for degradation by the 26S proteasome. Three components are involved in the target protein-ubiquitin conjugation process. Ubiquitin is first activated by forming a thiolester complex with the activation component E1; the activated ubiquitin is subsequently transferred to the ubiquitin-carrier protein E2, then from E2 to ubiquitin ligase E3 for final delivery to the epsilon-NH2 of the target protein lysine residue (1-3). Combinatorial interactions of different E2 and E3 proteins result in substrate specificity (4). Recent data suggest that activated E2 associates transiently with E3, and that the dissociation is a critical step for ubiqitination (5). Cullin homolog 1 (CUL1), the mammalian homolog of Cdc53 from yeast, is a molecular scaffold of the SCF (Skp1/CUL1/F-box) E3 ubiquitin ligase protein complex. Thus, CUL1 and its family members function in ubiquitin dependent proteolysis (6). In particular, CUL1 has been shown to mediate ubiquitin dependent degradation of p21 Waf1/Cip1, cyclin D and IkappaB-alpha (7,8).
For Research Use Only. Not For Use In Diagnostic Procedures.
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