CTPS1 Antibody #98287
Filter:
- WB
Western blot analysis of extracts from various cell lines using CTPS1 Antibody.
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | 78 |
| SOURCE | Rabbit |
Application Key:
- WB-Western Blotting
Species Cross-Reactivity Key:
- H-Human
- Related Products
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
CTPS1 Antibody recognizes endogenous levels of total CTPS1 protein. This antibody does not cross-react with CTPS2 protein.
Species Reactivity:
Human
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala340 of human CTPS1 protein. Antibodies are purified by peptide affinity chromatography.
Background
CTPS1 (cytidine triphosphate synthase 1 or CTP synthase 1) catalyzes the conversion of UTP to CTP through ATP-dependent amination (1). Research studies show that pyrimidine biosynthesis is upregulated in pancreatic cancer cell models of resistance to gemcitabine, a deoxycytidine analog. Increased pyrimidine biosynthesis leads to elevated levels of endogenous dCTP, which outcompetes gemcitabine from incorporating into DNA during replication and, therefore, reduces the anti-cancer effectiveness of gemcitabine. Induction of CTPS1 expression was observed in gemcitabine-resistant pancreatic cancer cell models. Gemcitabine resistance directly correlates with CTPS1 expression levels in various pancreatic cancer cell lines (2). Furthermore, CTPS1 expression was shown to increase in T cells upon T-cell receptor activation, suggesting a critical role of CTPS1 in the immune system (3).
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For Research Use Only. Not For Use In Diagnostic Procedures.
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