CSDE1 Antibody #62328
Filter:
- WB
Supporting Data
| REACTIVITY | H M R Mk |
| SENSITIVITY | Endogenous |
| MW (kDa) | 89 |
| SOURCE | Rabbit |
Application Key:
- WB-Western Blotting
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
- Mk-Monkey
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
CSDE1 Antibody recognizes endogenous levels of total CSDE1 protein.
Species Reactivity:
Human, Mouse, Rat, Monkey
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human CSDE1 protein. Antibodies are purified by peptide affinity chromatography.
Background
Originally described as the product of an active transcription unit located upstream of the N-RAS gene (1), CSDE1 (cold shock domain-containing protein E1) is a highly conserved RNA-binding protein containing five cold-shock domains (2,3). CSDE1 is mostly localized in the cytoplasm where it interacts with distinct protein complexes involved in the post-transcriptional regulation of numerous mRNAs in a dynamic and complex manner (4). CSDE1 plays a role in maintaining the undifferentiated state of human embryonic stem cells (hESCs), and its loss results in accelerated neural differentiation and neurogenesis, partially via post-transcriptional regulation of fatty acid binding protein 7 (FABP7) and vimentin (VIM) mRNAs (5). In human melanoma, increased CSDE1 protein expression has been shown to modulate the levels of pro-oncogenic factors such as vimentin or RAC1 and tumor suppressors such as PTEN, thus promoting tumor invasion and metastasis (6).
- Jeffers, M. et al. (1990) Nucleic Acids Res 18, 4891-9.
- Goroncy, A.K. et al. (2010) J Struct Funct Genomics 11, 181-8.
- Triqueneaux, G. et al. (1999) Nucleic Acids Res 27, 1926-34.
- Mihailovich, M. et al. (2010) Bioessays 32, 109-18.
- Ju Lee, H. et al. (2017) Nat Commun 8, 1456.
- Wurth, L. et al. (2016) Cancer Cell 30, 694-707.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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