CREB (D76D11) Rabbit mAb #4820

Filter:

WB
IP
IF
F
ChIP
C&T

Supporting Data

REACTIVITY	H M R Hm Mk Dm
SENSITIVITY	Endogenous
MW (kDa)	43
Source/Isotype	Rabbit IgG

Application Key:

WB-Western Blotting
IP-Immunoprecipitation
IF-Immunofluorescence
F-Flow Cytometry
ChIP-Chromatin Immunoprecipitation
C&T-CUT & Tag

Species Cross-Reactivity Key:

H-Human
M-Mouse
R-Rat
Hm-Hamster
Mk-Monkey
Dm-D. melanogaster

Product Information

Product Usage Information

For optimal ChIP results, use 5 μl of antibody and 10 μg of chromatin (approximately 4 x 10⁶ cells) per IP. This antibody has been validated using SimpleChIP^® Enzymatic Chromatin IP Kits.

Application	Dilution
Western Blotting	1:1000
Simple Western™	1:50 - 1:250
Immunoprecipitation	1:50
Immunofluorescence (Frozen)	1:400 - 1:1600
Immunofluorescence (Immunocytochemistry)	1:400 - 1:1600
Flow Cytometry (Fixed/Permeabilized)	1:200 - 1:800
Chromatin IP	1:100
CUT&Tag	1:50

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

CREB (D76D11) Rabbit mAb detects endogenous levels of total CREB-1 protein. The antibody does not cross-react with other ATF/CREB family members.

Species Reactivity:

Human, Mouse, Rat, Hamster, Monkey, D. melanogaster

Source / Purification

Monoclonal antibody is produced by immunizing animals with a full length GST-CREB fusion protein. The epitope has been mapped to residues surrounding Gly61.

Background

CREB is a bZIP transcription factor that activates target genes through cAMP response elements. CREB is able to mediate signals from numerous physiological stimuli, resulting in regulation of a broad array of cellular responses. While CREB is expressed in numerous tissues, it plays a large regulatory role in the nervous system. CREB is believed to play a key role in promoting neuronal survival, precursor proliferation, neurite outgrowth, and neuronal differentiation in certain neuronal populations (1-3). Additionally, CREB signaling is involved in learning and memory in several organisms (4-6). CREB is able to selectively activate numerous downstream genes through interactions with different dimerization partners. CREB is activated by phosphorylation at Ser133 by various signaling pathways, including Erk, Ca²⁺, and stress signaling. Some of the kinases involved in phosphorylating CREB at Ser133 are p90RSK, MSK, CaMKIV, and MAPKAPK-2 (7-9).

Database Links

UniProt ID: P16220

Entrez-Gene Id: 1385

限制使用

除非 CST 的合法授书代表以书面形式书行明确同意，否书以下条款适用于 CST、其关书方或分书商提供的书品。任何书充本条款或与本条款不同的客书条款和条件，除非书 CST 的合法授书代表以书面形式书独接受，否书均被拒书，并且无效。

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For Research Use Only. Not For Use In Diagnostic Procedures.

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