R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
COBRA1 (D6K9A) Rabbit mAb #14894
Filter:
- WB
- IP
- ChIP
Supporting Data
| REACTIVITY | H M R Mk |
| SENSITIVITY | Endogenous |
| MW (kDa) | 65 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
- ChIP-Chromatin Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
- Mk-Monkey
Product Information
Product Usage Information
For optimal ChIP and ChIP-seq results, use 10 μl of antibody and 10 μg of chromatin (approximately 4 x 106 cells) per IP. This antibody has been validated using SimpleChIP® Enzymatic Chromatin IP Kits.
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
| Chromatin IP | 1:50 |
| Chromatin IP-seq | 1:50 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
COBRA1 (D6K9A) Rabbit mAb recognizes endogenous levels of total COBRA1 protein.
Species Reactivity:
Human, Mouse, Rat, Monkey
The antigen sequence used to produce this antibody shares 100% sequence homology with the species listed here, but reactivity has not been tested or confirmed to work by CST. Use of this product with these species is not covered under our Product Performance Guarantee.
Species predicted to react based on 100% sequence homology:
Hamster, Bovine
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human COBRA1 protein.
Background
Negative Elongation Factor (NELF) consists of four subunits: WHSC2 (NELF-A), COBRA-1 (NELF-B), TH1L (NELF-C/D), and NELF-E (1). NELF, together with DRB-sensitivity inducing factor (DSIF), inhibits RNA Polymerase II (RNAPII) elongation resulting in RNAPII promoter proximal pausing, where it waits additional signaling to resume transcription (2,3). The release of RNAPII from promoter proximal pausing is a critical regulatory point during transcription and is signaled by positive transcription elongation factor (p-TEF-b) phosphorylation of both NELF and the carboxy-terminal domain (CTD) within the largest subunit of RNAPII (3,4). WHSC2 is thought to connect the NELF complex to RNAPII machinery, while NELF-E contains an RNA binding motif that is necessary for NELF function (1,5,6). TH1L, together with COBRA-1, are integral subunits that bring WHSC2 and NELF-E together in the NELF complex (1).
Cofactor of BRCA1 (COBRA1, NELF-B) was first identified as a factor that interacts with the BRCT domain of the tumor suppressor protein BRCA1 (7). COBRA1 is a modulator of ligand dependent and independent expression of estrogen receptor-α target genes (8,9). COBRA1 expression is reduced in metastatic and recurrent breast cancer, suggesting that low levels of COBRA1 in breast cancers may serve as an indicator for poor prognosis (10).
Cofactor of BRCA1 (COBRA1, NELF-B) was first identified as a factor that interacts with the BRCT domain of the tumor suppressor protein BRCA1 (7). COBRA1 is a modulator of ligand dependent and independent expression of estrogen receptor-α target genes (8,9). COBRA1 expression is reduced in metastatic and recurrent breast cancer, suggesting that low levels of COBRA1 in breast cancers may serve as an indicator for poor prognosis (10).
- Narita, T. et al. (2003) Mol Cell Biol 23, 1863-73.
- Nechaev, S. and Adelman, K. (2011) Biochim Biophys Acta 1809, 34-45.
- Yamaguchi, Y. et al. (1999) Cell 97, 41-51.
- Buratowski, S. (2009) Mol Cell 36, 541-6.
- Yamaguchi, Y. et al. (2001) Science 293, 124-7.
- Yamaguchi, Y. et al. (2002) Mol Cell Biol 22, 2918-27.
- Ye, Q. et al. (2001) J Cell Biol 155, 911-21.
- Aiyar, S.E. et al. (2004) Genes Dev 18, 2134-46.
- Aiyar, S.E. et al. (2007) Oncogene 26, 2543-53.
- Sun, J. et al. (2008) J Cell Biochem 103, 1798-807.
限制使用
除非 CST 的合法授书代表以书面形式书行明确同意，否书以下条款适用于 CST、其关书方或分书商提供的书品。 任何书充本条款或与本条款不同的客书条款和条件，除非书 CST 的合法授书代表以书面形式书独接受， 否书均被拒书，并且无效。
专品专有“专供研究使用”的专专或专似的专专声明， 且未专得美国食品和专品管理局或其他外国或国内专管机专专专任何用途的批准、准专或专可。客专不得将任何专品用于任何专断或治专目的， 或以任何不符合专专声明的方式使用专品。CST 专售或专可的专品提供专作专最专用专的客专，且专用于研专用途。将专品用于专断、专防或治专目的， 或专专售（专独或作专专成）或其他商专目的而专专专品，均需要 CST 的专独专可。客专：(a) 不得专独或与其他材料专合向任何第三方出售、专可、 出借、捐专或以其他方式专专或提供任何专品，或使用专品制造任何商专专品，(b) 不得复制、修改、逆向工程、反专专、 反专专专品或以其他方式专专专专专品的基专专专或技专，或使用专品开专任何与 CST 的专品或服专专争的专品或服专， (c) 不得更改或专除专品上的任何商专、商品名称、徽专、专利或版专声明或专专，(d) 只能根据 CST 的专品专售条款和任何适用文档使用专品， (e) 专遵守客专与专品一起使用的任何第三方专品或服专的任何专可、服专条款或专似专专
For Research Use Only. Not For Use In Diagnostic Procedures.
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