R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
CLEC2D (E4K1H) Rabbit mAb #24276
Filter:
- WB
- IP
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | 20-30 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
CLEC2D (E4K1H) Rabbit mAb recognizes endogenous levels of total CLEC2D protein. Based on the antigen sequence, this antibody is predicted to recognize multiple isoforms of CLEC2D.
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human CLEC2D protein.
Background
C-type lectin domain family 2 member D (CLEC2D), also known as lectin-like transcript 1 (LLT1) and osteoclast inhibitory lectin (OCIL), is a type II transmembrane C-type lectin receptor primarily expressed upon immune cell activation in T cells, B cells, NK cells, and plasmacytoid and monocyte-derived dendritic cells (1-4). CLEC2D interacts with NKR-P1A (KLRB1, CD161) on NK cells and some subsets of T cells, inhibiting NK cell cytotoxicity while alternatively inducing proliferation and IFNγ production in T cells (2-4). CLEC2D is also expressed by osteoblasts, inhibiting osteoclast formation and bone resorption (5,6). Multiple isoforms of CLEC2D produced by alternative splicing have been identified (7).
- Boles, K.S. et al. (1999) Immunogenetics 50, 1-7.
- Germain, C. et al. (2011) J Biol Chem 286, 37964-37975.
- Rosen, D.B. et al. (2005) J Immunol 175, 7796-9.
- Rosen, D.B. et al. (2008) J Immunol 180, 6508-17.
- Hu, Y.S. et al. (2004) J Bone Miner Res 19, 89-99.
- Gange, C.T. et al. (2004) J Biol Chem 279, 29043-9.
- Germain, C. et al. (2010) J Biol Chem 285, 36207-15.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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