Cleaved PARP (Asp214) Antibody #9544
Filter:
- WB
Western blot analysis of extracts from HeLa cells and serum starved WEHI-231 cells, untreated or treated with staurosporine (1 µM, 3 hrs), using Cleaved PARP (Asp214) Antibody #9544 (upper) or PARP (46D11) Rabbit mAb #9532 (lower).
To Purchase # 9544
Cat. #
Size
Price
Inventory
9544T
20 µl
¥2,120
库存充足
9544S
100 µl
¥5,220
库存紧张
Supporting Data
| REACTIVITY | H M |
| SENSITIVITY | Endogenous |
| MW (kDa) | 89 |
| SOURCE | Rabbit |
Application Key:
- WB-Western Blotting
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- Related Products
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
Cleaved PARP (Asp214) Antibody detects endogenous levels of the large fragment (89 kDa) of PARP1 resulting from caspase cleavage. The antibody does not recognize full length PARP1 or other PARP isoforms.
Species Reactivity:
Human, Mouse
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to carboxy-terminal residues surrounding Asp214 in mouse PARP. Antibodies are purified by protein A and peptide affinity chromatography.
Background
PARP, a 116 kDa nuclear poly (ADP-ribose) polymerase, appears to be involved in DNA repair in response to environmental stress (1). This protein can be cleaved by many ICE-like caspases in vitro (2,3) and is one of the main cleavage targets of caspase-3 in vivo (4,5). In human PARP, the cleavage occurs between Asp214 and Gly215, which separates the PARP amino-terminal DNA-binding domain (24 kDa) from the carboxy-terminal catalytic domain (89 kDa) (2,4). PARP helps cells to maintain their viability; cleavage of PARP facilitates cellular disassembly and serves as a marker of cells undergoing apoptosis (6).
(This product is sold under license from Promega Corp., U.S. Patent No. 6,350,452.)
(This product is sold under license from Promega Corp., U.S. Patent No. 6,350,452.)
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For Research Use Only. Not For Use In Diagnostic Procedures.
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