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Render Timestamp: 2025-03-04T16:49:50.965Z
Commit: 7500bcdc731e9059bbdfbdbe9e72caa896e426e8
XML generation date: 2025-02-06 11:42:48.613
Product last modified at: 2025-02-07T08:01:26.223Z
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PDP - Template Name: Matched Antibody Pair
PDP - Template ID: *******446e1e7

Cleaved Gasdermin D (Asp275) Matched Antibody Pair #17710

Filter:
  • ELISA
ELISA Image 1: Cleaved Gasdermin D (Asp275) Matched Antibody Pair
Data using Cleaved Gasdermin D (Asp275) Matched Antibody Pair #17710 are shown. The two antibodies function as a capture-target-detection sandwich (#69312 and #37349, respectively) to detect protein expression in positive and negative controls.

To Purchase # 17710

Supporting Data

REACTIVITY H
Application Key:
  • ELISA-ELISA 
Species Cross-Reactivity Key:
  • H-Human 
  • Product Includes
  • Related Products

Product Information

Product Usage Information

Matched Antibody Pairs consist of capture and detection antibodies that bind to non-overlapping epitopes. For specific identification of the capture and detection antibodies in this pair, please refer to the data figure caption. Optimal dilutions/concentrations should be determined by the end user.

Formulation

Supplied in 1X PBS (10 mM Na2HPO4, 3 mM KCl, 2 mM KH2PO4, and 140 mM NaCl (pH 7.8)). BSA and Azide Free.

Storage

Store at -20ºC. This product will freeze at -20ºC so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance.

Product Description

The Cleaved Gasdermin D (Asp275) Matched Antibody Pair is ideal for use with immunoassay technologies and high-throughput ELISA platforms requiring antibody pairs with specialized or custom antibody labeling. Labels include fluorophores, lanthanides, biotin, and beads. Platforms requiring conjugated Matched Antibody Pairs include MSD, Quanterix Simoa, Alpha Technology (AlphaScreen, AlphaLISA, LANCE, HTRF), and Luminex.

Learn how Matched Antibody Pairs move your projects forward, faster at cst-science.com/matched-antibody-pairs.

Background

Gasdermin D (GSDMD), a member of the gasdermin family that includes GSDMA, GSDMB, and GSDMC, has been reported to have a critical role as a downstream effector of pyroptosis (1,2). Pyroptosis is a lytic type of cell death triggered by inflammasomes, multiprotein complexes assembled in response to pathogen-associated molecular patterns (PAMPs) or danger-associated molecular patterns (DAMPs) that result in the activation of caspase-1 and subsequent cleavage of pro-inflammatory cytokines IL-1β and IL-18 (3). Gasdermin D was identified by two independent groups as a substrate of inflammatory caspases, caspase-1 and caspase-11/4/5, producing two fragments: GSDMD-N and GSDMD-C. Cleavage results in release of an intramolecular inhibitory interaction between the N- and C-terminal domains, allowing the N-terminal fragment GSDMD-N to initiate pyroptosis through the formation of pores on the plasma membrane (4-7).
For Research Use Only. Not For Use In Diagnostic Procedures.
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