R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
Choline Kinase α (D5X9W) Rabbit mAb #13422
Filter:
- WB
- IP
Supporting Data
| REACTIVITY | H Mk |
| SENSITIVITY | Endogenous |
| MW (kDa) | 50 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
- Mk-Monkey
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:100 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
Choline Kinase α (D5X9W) Rabbit mAb recognizes endogenous levels of total choline kinase α protein. Based on the antigen sequence, this antibody is not expected to recognize choline kinase β.
Species Reactivity:
Human, Monkey
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro85 of human choline kinase α protein.
Background
Choline kinase (ChoK) catalyzes the phosphorylation of choline, a key step in the biosynthesis of the membrane phospholipid phosphatidylcholine. At least three ChoK isoforms exist in mammalian cells, α-1, α-2, and β. The two α isoforms are transcribed from the same CHKA gene as splice variants, while the β isoform resides on a separate CHKB gene (reviewed in 1).
Research studies indicate that ChoKα levels affect signaling through MAPK and Akt pathways (2,3). Investigators have shown that ChoKα plays a role in proliferation and carcinogenesis and is highly expressed/activated in human cancers (4-7). Additional research studies suggest ChoKα may be a potential target for cancer therapy (8).
Research studies indicate that ChoKα levels affect signaling through MAPK and Akt pathways (2,3). Investigators have shown that ChoKα plays a role in proliferation and carcinogenesis and is highly expressed/activated in human cancers (4-7). Additional research studies suggest ChoKα may be a potential target for cancer therapy (8).
- Janardhan, S. et al. (2006) Curr Med Chem 13, 1169-86.
- Yalcin, A. et al. (2010) Oncogene 29, 139-49.
- Chua, B.T. et al. (2009) Mol Cancer 8, 131.
- Ramírez de Molina, A. et al. (2002) Oncogene 21, 4317-22.
- Ramírez de Molina, A. et al. (2007) Lancet Oncol 8, 889-97.
- Hernando, E. et al. (2009) Oncogene 28, 2425-35.
- Miyake, T. and Parsons, S.J. (2012) Oncogene 31, 1431-41.
- Bañez-Coronel, M. et al. (2008) Curr Cancer Drug Targets 8, 709-19.
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For Research Use Only. Not For Use In Diagnostic Procedures.
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