R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
Chitotriosidase-1/CHIT1 (E4X4I) Rabbit mAb #44627
Filter:
- WB
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | 50 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Simple Western™ | 1:10 - 1:50 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
Chitotriosidase-1/CHIT1 (E4X4I) Rabbit mAb recognizes endogenous levels of total Chitotriosidase-1/CHIT1 protein.
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser406 of human Chitotriosidase-1/CHIT1 protein.
Background
Chitin is an essential biopolymer structural component of many organisms, including arthropods, fungi, and parasites. Chitinases are part of the 18-glycoside hydrolase (18-GH) family of proteins that act to bind and degrade chitin. In mammals, this function provides defense against chitin-containing pathogens. Chitotriosidase-1, also known as Chitinase-1 or CHIT1, is the most abundant of these enzymes. CHIT1 consists of an N-terminal catalytic domain, a C-terminal chitin-binding domain, and a short hinge region that connects the two. CHIT1 activity is implicated in several inflammatory signaling pathways and is considered a sensitive biomarker for activated immune cells. It is produced and secreted by macrophages, microglia, and neutrophils in normal and inflammatory conditions. Elevated levels of CHIT1 in serum is a suggested biomarker for Gaucher's disease, atherosclerosis, pulmonary fibrosis, and some cancers. Elevated CSF levels of CHIT1 is also a suggested biomarker for amyotrophic lateral sclerosis (ALS) and other neurodegenerative diseases (1-5).
- Kanneganti, M. et al. (2012) J Epithel Biol Pharmacol 5, 1-9.
- Lee, C.M. et al. (2019) Life Sci Alliance 2, e201900350. doi: 10.26508/lsa.201900350.
- Majewski, S. et al. (2022) Front Immunol 13, 760776.
- Russo, C. et al. (2023) Int J Mol Sci 24, 6301. doi: 10.3390/ijms24076301.
- Mishra, P.S. et al. (2017) J Neuroinflammation 14, 251.
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For Research Use Only. Not For Use In Diagnostic Procedures.
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