R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
ChAT (E4F9G) Rabbit mAb #27269
Filter:
- WB
Supporting Data
| REACTIVITY | H M R |
| SENSITIVITY | Endogenous |
| MW (kDa) | 71 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
ChAT (E4F9G) Rabbit mAb recognizes endogenous levels of total human, mouse, and rat ChAT protein.
Species Reactivity:
Human, Mouse, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the carboxy terminus of human ChAT protein.
Background
Choline O-acetyltransferase (ChAT) catalyzes the synthesis of the neurotransmitter acetylcholine (ACh) from acetyl CoA and choline in the central and peripheral nervous system (1). In mammals, ACh is the primary neurotransmitter used at the neuromuscular junction, released by motor neurons to activate muscles. Neurons that release ACh are known as cholinergic neurons, which express high levels of ChAT. In addition to motor neurons, cholinergic neurons are present in the brain, particularly in the basal forebrain as well as other regions of the brain, including striatal interneurons. Reduction in ChAT, indicative of degeneration of cholinergic neurons, is correlated with cognitive decline in several neurodegenerative diseases, including Alzheimer’s disease (2).
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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