R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
CELSR2 (D2M9H) XP® Rabbit mAb (BSA and Azide Free) #19983
Filter:
- WB
- IHC
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | 320 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IHC-Immunohistochemistry
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
This product is the carrier free version of product #47061. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol.
This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us. Optimal dilutions/concentrations should be determined by the end user.
BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity.
This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us. Optimal dilutions/concentrations should be determined by the end user.
BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity.
Formulation
Supplied in 1X PBS (10 mM Na2HPO4, 3 mM KCl, 2 mM KH2PO4, and 140 mM NaCl (pH 7.8)). BSA and Azide Free.
For standard formulation of this product see product #47061
For standard formulation of this product see product #47061
Storage
Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance.
Specificity / Sensitivity
CELSR2 (D2M9H) XP® Rabbit mAb (BSA and Azide Free) recognizes endogenous levels of total CELSR2 protein.
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding His1781 of human CELSR2 protein.
Background
CELSR2 (cadherin EGF LAG seven-pass G-type receptor, also known as flamingo homolog 3 or epidermal growth factor-like protein 2) is a member of the flamingo subfamily of non-classical cadherins, part of the cadherin superfamily. CELSR2 is a 7-transmembrane helix receptor that contains nine cadherin-like domains, seven EGF-like repeats, and 2 laminin A G-type repeats (1). It shares structural characteristics of both an adhesion molecule and a G protein-coupled receptor, suggesting putatives roles in both cell-cell adhesion and juxtacrine signaling. It's function has been associated with dendrite morphogenesis (2), neural plate anterior-posterior pattern formation (3), and regulation of transcription via the Wnt signaling pathway (4). In a loss-of-function mouse model, Celsr2 deletion resulted in defects in the planar organization of ependymal cilia, leading to defective cerebrospinal fluid dynamics and hydrocephalus (5). In humans, SNPs in the CELSR2 gene cluster on chromosome 1 have been associated with enhanced risk of coronary artery disease (6).
- Vincent, J.B. et al. (2000) DNA Res 7, 233-5.
- Shima, Y. et al. (2004) Dev Cell 7, 205-16.
- Devenport, D. and Fuchs, E. (2008) Nat Cell Biol 10, 1257-68.
- Qu, Y. et al. (2014) Proc Natl Acad Sci U S A 111, E2996-3004.
- Tissir, F. et al. (2010) Nat Neurosci 13, 700-7.
- Zhou, Y.J. et al. (2015) Int J Clin Exp Pathol 8, 9543-51.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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