R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
CEACAM8 (D2F5U) Rabbit mAb #61572
Filter:
- WB
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | 200 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
CEACAM8 (D2F5U) Rabbit mAb recognizes endogenous levels of total CEACAM8 protein. This antibody does not cross-react with CEACAM1 or CEACAM6 proteins.
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the carboxy terminus of human CEACAM8 protein.
Background
Carcinoembryonic antigen-related cell adhesion molecule 8 (CEACAM8), also known as CD66b, is a member of the CEA-related cell-adhesion molecule (CEACAM) family (1). CEACAMs bind to themselves and other family members to carry out numerous cellular functions, including proliferation, signaling, differentiation, tumor suppression, and survival (2). CEACAM8 is a single-chain, glycosylphosphatidylinositol (GPI)-anchored, highly glycosylated protein that under normal conditions is expressed almost exclusively on granulocytes (3). Heterophilic interactions of CEACAM8 with other surface molecules, such as CEACAM6, have been shown to be involved in regulating cell adhesion in a calcium-independent manner (4). As such, CEACAM8 has demonstrated utility as a marker of neutrophil and eosinophil activation (5,6) and in pathological conditions is shown to be highly expressed in primary myelofibrosis and acute lymphoblastic leukemias (7,8). Assessment of CEACAM8 positive tumor-infiltrating neutrophils has demonstrated value as a prognostic factor in multiple cancer types (9,10).
- Kuespert, K. et al. (2006) Curr Opin Cell Biol 18, 565-71.
- Bonsor, D.A. et al. (2015) Proc Natl Acad Sci U S A 112, 13561-6.
- Zhao, L. et al. (2004) J Immunol Methods 293, 207-14.
- Kuroki, M. et al. (2001) J Leukoc Biol 70, 543-50.
- Yamanaka, T. et al. (1996) Protein Expr Purif 7, 438-46.
- Yoon, J. et al. (2007) J Immunol 179, 8454-62.
- Hasselbalch, H.C. et al. (2011) Leuk Res 35, 1330-4.
- Lasa, A. et al. (2008) Ann Hematol 87, 205-11.
- Waldmann, T.A. (1987) Adv Exp Med Biol 213, 129-37.
- Zhang, H. et al. (2018) Ann Surg 267, 311-8.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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