R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
CDK2AP1 (E9D7P) Rabbit mAb #89650
Filter:
- WB
- IP
- ChIP
Supporting Data
| REACTIVITY | H Mk |
| SENSITIVITY | Endogenous |
| MW (kDa) | 12 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
- ChIP-Chromatin Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
- Mk-Monkey
Product Information
Product Usage Information
For optimal ChIP results, use 10 μL of antibody and 10 μg of chromatin (approximately 4 × 106 cells) per IP. This antibody has been validated using SimpleChIP® Enzymatic Chromatin IP Kits.
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:200 |
| Chromatin IP | 1:50 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
CDK2AP1 (E9D7P) Rabbit mAb recognizes endogenous levels of total CDK2AP1 protein.
Species Reactivity:
Human, Monkey
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro12 of human CDK2AP1 protein.
Background
Cyclin-dependent kinase 2-associated protein 1 (CDK2AP1) was first identified as a tumor suppressor in oral cancer and is a member of the nucleosome remodeling and histone deacetylase (NuRD) complex (1-4). Also known as p12 or DOC-1, CDK2AP1 binds to unphosphorylated CDK2 and DNA polymerase alpha/primase to negatively regulate their activities (5,6). CDK2AP1 also plays a role in the silencing of Oct-4 during stem cell differentiation (7). Low CDK2AP1 expression in oral cancers is associated with poor prognosis (8). Lower expression has also shown to be resistant to cisplatin treatment (9). In addition to oral cancers, CDK2AP1 plays a role in other tumor types, including colorectal, testicular, and ovarian cancers (10,11).
- Todd, R. et al. (1995) FASEB J 9, 1362-70.
- Daigo, Y. et al. (1997) Genes Chromosomes Cancer 20, 204-7.
- Tsuji, T. et al. (1998) J Biol Chem 273, 6704-9.
- Spruijt, C.G. et al. (2010) Mol Biosyst 6, 1700-6.
- Shintani, S. et al. (2000) Mol Cell Biol 20, 6300-7.
- Matsuo, K. et al. (2000) FASEB J 14, 1318-24.
- Deshpande, A.M. et al. (2009) J Biol Chem 284, 6043-7.
- Shintani, S. et al. (2001) Clin Cancer Res 7, 2776-82.
- Kim, Y. et al. (2005) Oncogene 24, 407-18.
- Yuan, Z. et al. (2003) Oncogene 22, 6304-10.
- Figueiredo, M.L. et al. (2006) Mol Reprod Dev 73, 987-97.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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