R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
CDK10 (D8Z7Z) Rabbit mAb #36106
Filter:
- WB
Supporting Data
| REACTIVITY | H M R |
| SENSITIVITY | Endogenous |
| MW (kDa) | 40 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
CDK10 (D8Z7Z) Rabbit mAb recognizes endogenous levels of total CDK10 protein. This antibody is not expected to react with CDK10 N-terminal deletion isoforms.
Species Reactivity:
Human, Mouse, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val24 of human CDK10 protein.
Background
Cyclin Dependent Kinase 10 (CDK10) is a Cdc2-related protein kinase that binds to and inhibits the transactivation activity of the transcription factor Ets2 (1).
CDK10 is activated by cyclin M, which is mutated and unable to activate CDK10 in the human developmental disorder, STAR syndrome. Phosphorylation of Ets2 by CDK10/Cyclin M leads to degradation of Ets2 by the proteasome (2). CDK10 also plays a role in the development of the zebrafish nervous system (3). Studies have shown that expression of CDK10, which is modulated by promoter hypermethylation, is decreased in human cancer (4-6). Further, studies show that CDK10 expression in breast cancer affects response to tamoxifen (7), and is correlated with disease progression (8). CDK10 regulates the expression of c-RAF, and signaling through the MAPK pathway (2-3, 6-7).
CDK10 is activated by cyclin M, which is mutated and unable to activate CDK10 in the human developmental disorder, STAR syndrome. Phosphorylation of Ets2 by CDK10/Cyclin M leads to degradation of Ets2 by the proteasome (2). CDK10 also plays a role in the development of the zebrafish nervous system (3). Studies have shown that expression of CDK10, which is modulated by promoter hypermethylation, is decreased in human cancer (4-6). Further, studies show that CDK10 expression in breast cancer affects response to tamoxifen (7), and is correlated with disease progression (8). CDK10 regulates the expression of c-RAF, and signaling through the MAPK pathway (2-3, 6-7).
- Kasten, M. and Giordano, A. (2001) Oncogene 20, 1832-8.
- Guen, V.J. et al. (2013) Proc Natl Acad Sci U S A 110, 19525-30.
- Yeh, C.W. et al. (2013) J Biol Chem 288, 27927-39.
- You, Y. et al. (2013) Cell Oncol (Dordr) 36, 323-31.
- Zhong, X.Y. et al. (2012) Gene 498, 68-74.
- Yu, J.H. et al. (2012) Oncol Rep 27, 1266-76.
- Iorns, E. et al. (2008) Cancer Cell 13, 91-104.
- You, Y. et al. (2015) Cell Oncol (Dordr) 38, 485-91.
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For Research Use Only. Not For Use In Diagnostic Procedures.
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