R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
CD9 (E3O1B) Rabbit mAb #99950
Filter:
- WB
- F
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | 22-27 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- F-Flow Cytometry
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Simple Western™ | 1:10 - 1:50 |
| Flow Cytometry (Live) | 1:100 - 1:400 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
CD9 (E3O1B) Rabbit mAb recognizes endogenous levels of total CD9 protein.
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Thr175 of human CD9 protein.
Background
The CD9 antigen belongs to the tetraspanin family of cell surface glycoproteins, and is characterized by four transmembrane domains, one short extracellular domain (ECL1), and one long extracellular domain (ECL2). Tetraspanins interact with a variety of cell surface proteins and intracellular signaling molecules in specialized tetraspanin-enriched microdomains (TEMs), where they mediate a range of processes including adhesion, motility, membrane organization, and signal transduction (1). Research studies demonstrate that CD9 expression on the egg is required for gamete fusion during fertilization (2-4). CD9 was also shown to play a role in dendritic cell migration, megakaryocyte differentiation, and homing of cord blood CD34+ hematopoietic progenitors to the bone marrow (5-7). In addition, downregulation of CD9 expression is associated with poor prognosis and progression of several types of cancer (8-10). Additional research identified CD9 as an abundant component of exosomes, and may play some role in the fusion of these secreted membrane vesicles with recipient cells (11).
- Hemler, M.E. (2005) Nat Rev Mol Cell Biol 6, 801-11.
- Le Naour, F. et al. (2000) Science 287, 319-21.
- Miyado, K. et al. (2000) Science 287, 321-4.
- Kaji, K. et al. (2000) Nat Genet 24, 279-82.
- Mantegazza, A.R. et al. (2004) Blood 104, 1183-90.
- Clay, D. et al. (2001) Blood 97, 1982-9.
- Leung, K.T. et al. (2011) Blood 117, 1840-50.
- Miyake, M. et al. (1995) Cancer Res 55, 4127-31.
- Higashiyama, M. et al. (1995) Cancer Res 55, 6040-4.
- Uchida, S. et al. (1999) Br J Cancer 79, 1168-73.
- Théry, C. et al. (1999) J Cell Biol 147, 599-610.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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