Render Target: SSR
Render Timestamp: 2025-03-15T05:08:40.934Z
Commit: 9fc0f116116d9da247dc8ddd4e5fe811153412e1
XML generation date: 2024-08-01 15:31:11.827
Product last modified at: 2025-03-03T19:15:10.171Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

CD86 (E5W6H) Rabbit mAb #19589

Filter:
  • WB
  • IHC
Western Blotting Image 1: CD86 (E5W6H) Rabbit mAb
Western blot analysis of extracts from J774A.1 cells, Neuro-2a cells, and mouse spleen tissue using CD86 (E5W6H) Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).

To Purchase # 19589

Cat. #

Size

Price

Inventory

19589T
20 µl
¥2,030

无库存

19589S
100 µl
¥4,550

库存充足

Supporting Data

REACTIVITY M
SENSITIVITY Endogenous
MW (kDa) 60-85
Source/Isotype Rabbit IgG
Application Key:
  • WB-Western Blotting 
  • IHC-Immunohistochemistry 
Species Cross-Reactivity Key:
  • M-Mouse 
  • Related Products

Product Information

Product Usage Information

Application Dilution
Western Blotting 1:1000
IHC Leica Bond 1:200 - 1:800
Immunohistochemistry (Paraffin) 1:100 - 1:400

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

For a carrier free (BSA and azide free) version of this product see product #20018.

Protocol

Specificity / Sensitivity

CD86 (E5W6H) Rabbit mAb recognizes endogenous levels of total CD86 protein.

Species Reactivity:

Mouse

Source / Purification

Monoclonal antibody is produced by immunizing animals with mouse CD86 recombinant protein.

Background

CD80 (B7-1, BB1) and CD86 (B7-2, B70) are members of the B7 family of cell surface ligands that regulate T cell activation and immune responses. CD80 is expressed on activated antigen presenting cells, including dendritic cells, B cells, monocytes, and macrophages. CD86 is expressed on resting monocytes, dendritic cells, activated B lymphocytes, and can be further upregulated in the presence of inflammation (1-3). CD80 and CD86 are ligands for CD28, which functions as a T cell costimulatory receptor. Interaction of CD28 with CD80 or CD86 provides the second signal required for naïve T cell activation, T cell proliferation, and acquisition of effector functions (3-7). Alternatively, CD80 and CD86 also act as ligands to CTLA-4, which results in the downregulation of T cell activity (3,7-9).
For Research Use Only. Not For Use In Diagnostic Procedures.
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